DNA/RNA Sequence Manipulation in R

This R script provides functionality for manipulating DNA and RNA sequences, including generating reverse sequences, complementary sequences, and reverse-complementary sequences.

Key Features

• Complementary Base Pairing: Follows the standard complementary base pairing rules for DNA and RNA:
  • DNA: A -> T, C -> G, G -> C, T -> A
  • RNA: A -> U, C -> G, G -> C, T -> A
• Reverse Sequence Generation: Generates the reverse sequence of a given DNA or RNA sequence.
• Complementary Sequence Generation: Computes the complementary sequence of a given DNA sequence.
• Reverse-Complementary Sequence Generation: Produces the reverse-complementary sequence of a given DNA or RNA sequence.

Usage

1. Define the input DNA/RNA sequence.
2. Run the script to generate the desired sequences.

Functions

• generate_reverse_sequence(sequence): Generates the reverse sequence of the input sequence.
• generate_complementary_sequence(sequence): Generates the complementary sequence of the input DNA sequence.
• generate_reverse_complementary_sequence(sequence): Generates the reverse-complementary sequence of the input sequence.

Note

• The script assumes valid input sequences containing only valid nucleotide characters (A, T, C, G, U).
• Any non-nucleotide characters in the input sequence will be treated as unknown bases and flagged during processing.

Credits

This script was adapted from a Coursera course on Reverse and complement nucleic acid sequences (DNA, RNA) using R.