DAPI_NEUN_ORF1P

• Developed for: Tom
• Team: Fuchs
• Date: July 2023
• Software: Fiji

Images description

3D images taken on a spinning-disk with a x40 objective

3 channels:

1. CSU_405: nuclei
2. CSU_642: NeuN cells
3. CSU_561: ORF1p cells

Plugin description

• Detect DAPI nuclei, NeuN and ORF1p cells with Cellpose
• Compute their colocalization
• For each cell, give various intensity measurements in its nucleus, cytoplasm and entire mask

Dependencies

• 3DImageSuite Fiji plugin
• Cellpose conda environment + cyto2 model

Version history

Version 1 released on July 11, 2023.