PML_Partners_FixedCells

• Developed for: Pierre
• Team: De Thé
• Date: October 2022
• Software: Fiji

Images description

3D images taken with a x60 objective

3 channels:

1. Alexa Fluor 647: Partner foci (non mandatory)
2. Alexa Fluor 568: PML foci
3. DAPI: DAPI nuclei

Plugin description

• Find DAPI nuclei with StarDist
• Detect PML foci in each nucleus
• If Partner channel is provided, detect Partner foci in each nucleus and compute their colocalization with PML foci

Dependencies

• 3DImageSuite Fiji plugin
• CLIJ Fiji plugin
• StarDist conda environment + StandardFluo.zip and pmls2.zip (homemade) models

Version history

Version 1 released on October 26, 2022.