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How to get everyone sequence clonotype ? #65

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shwdmsh opened this issue Jul 22, 2021 · 0 comments
Open

How to get everyone sequence clonotype ? #65

shwdmsh opened this issue Jul 22, 2021 · 0 comments
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@shwdmsh
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shwdmsh commented Jul 22, 2021

Hi, I start to learn IGoR software.I use the command as follows:
(1).igor -set_wd $WDPATH -batch foo -read_seqs ./demo/demo.fasta
(2).igor -set_wd $WDPATH -batch foo -species human -chain IGL -align --all -threads 25
(3).igor -set_wd $WDPATH -batch foo -species human -chain IGL -evaluate -output --scenarios 10
(4).igor -set_wd $WDPATH -batch bar -species human -chain IGL -generate 100
and I get the foo_output:
seq_index;scenario_rank;scenario_proba_cond_seq;GeneChoice_V_gene_Undefined_side_prio7_size69;GeneChoice_J_gene_Undefined_side_prio6_size7;Deletion_V_gene_Three_prime_prio5_size21;Deletion_J_gene_Five_prime_prio5_size23;Insertion_VJ_gene_Undefined_side_prio4_size41;DinucMarkov_VJ_gene_Undefined_side_prio3_size16;Mismatches
39959;1;1;(4);(3);(8);(7);(4);(1,3,1,3);(1,2,3,5,7,8,9,11,13,14,15,16,18,19,20,125,156)
38201;1;0.382984;(41);(2);(2);(4);(0);();(0,2,3,4,6,7,8,9,12,14,17,18,19,48,58,67,166)
38201;2;0.245643;(41);(1);(2);(4);(0);();(0,2,3,4,6,7,8,9,12,14,17,18,19,48,58,67,166)
38201;3;0.193471;(41);(2);(2);(5);(1);(3);(0,2,3,4,6,7,8,9,12,14,17,18,19,48,58,67,166)

How could I get everyone sequence clonotype information,such as:

<style> </style>
clonotype identifier representative query sequence name count frequency (%) CDR3 nucleotide sequence CDR3 amino acid sequence productive status chain type V gene D gene J gene
1a number:1_length:188_4051 7 0.0241 CAGTCCTATGACAGCAGCCTGAGTGGTGCGGTG QSYDSSLSGAV No VL IGLV1-4001,IGLV1-4002 N/A IGLJ3*02
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