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Simultaneous lineage TRacking and EXpression profiling of single cells using RNA-seq (modified)

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Overview

Modified TREX pipeline, with only trex run10x kept.

Example of run

trex run10x \
  --chromosome <name_of_added_sequence> \
  --start <start_position> \
  --end <end_position> \
  --delete \
  --output <output_folder> \
  --amplicon <path_to_Cell_Ranger_amp_alignment> \
  <path_to_Cell_Ranger_alignment>

Additionally to the original pipeline, .h5ad-file with the result of barcode calling per each cell will be generated.

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Simultaneous lineage TRacking and EXpression profiling of single cells using RNA-seq (modified)

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  • Python 99.2%
  • Shell 0.8%