Modified TREX pipeline, with only trex run10x
kept.
trex run10x \
--chromosome <name_of_added_sequence> \
--start <start_position> \
--end <end_position> \
--delete \
--output <output_folder> \
--amplicon <path_to_Cell_Ranger_amp_alignment> \
<path_to_Cell_Ranger_alignment>
Additionally to the original pipeline, .h5ad-file with the result of barcode calling per each cell will be generated.