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Code for exploration of non-coding elements in single-cell RNA seq data

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single cell Transcriptionally Active Regions (scTARs)

Transcriptionally Active Regions are regions of the genome (including all introns, exons, and non-coding elements of the genome) which get transcribed into mRNA. Single cell sequencing technology has enabled detection of these transcripts at a cellular level, which has opened up the huge potential to study cell-type specific coding and non-coding elements in the genome.

This repository contains code for generating a counts matrix for non-coding elements from 10x single cell sequencing BAM files, which is a specially challenging problem due to lack of an annotation file for alignment.

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Code for exploration of non-coding elements in single-cell RNA seq data

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