Skip to content

Commit

Permalink
Clean up lifting docs
Browse files Browse the repository at this point in the history
- closes SomaLogic#82
  • Loading branch information
stufield committed Feb 22, 2024
1 parent 6c8ec23 commit 2fe5009
Show file tree
Hide file tree
Showing 2 changed files with 92 additions and 44 deletions.
67 changes: 45 additions & 22 deletions R/lift-adat.R
Original file line number Diff line number Diff line change
Expand Up @@ -3,38 +3,61 @@
#' The SomaScan platform continually improves its technical processes
#' between assay versions; from changing reagents, liquid handling equipment,
#' and increased analyte content. However, these upgrades can result in
#' minute differences in RFU space for a given analyte, requiring a calibration
#' minute measurement differences for a given analyte, requiring a calibration
#' (aka "lifting" or "bridging") to bring RFUs into a comparable space.
#' This is accomplished by applying an analyte-specific scalar to each analyte
#' RFU (ADAT column). The scalar values themselves are typically provided
#' via `*.xlsx` file, which can be parsed via [read_annotations()]. See Details.
#' This is accomplished by applying an analyte-specific scalar
#' (i.e. a linear transformation) to each analyte RFU (ADAT column).
#' The scalar values themselves are provided in a SomaScan annotations file
#' (`*.xlsx`), which can be parsed via [read_annotations()].
#'
#' Lifting between various versions requires a specific
#' annotations file containing scalars specific to desired lifting direction.
#' For example, to "lift" between `v4.1` -> `v4.0`, you *must* be working
#' with SomaScan data in `v4.1` space and an annotations file containing
#' scalars to convert __to__ `v4.0`.
#' Likewise, "lifting" from `v4.0` -> `v4.1` requires
#' a separate annotations file and a `soma_adat` from SomaScan `v4.0`.
#' Lifting between SomaScan versions no longer requires an
#' annotations file containing scalars. We now enable users to pass
#' a `bridge =` parameter, indicating the direction of the bridge.
#' For example, to "lift" between `11k` -> `7k`, you _must_ be acting on
#' SomaScan data in `11k` RFU space and would pass `bridge = "11k_to_7k"`.
#' Likewise, "lifting" from `7k` -> `5k` requires `bridge = "7k_to_5k"`.
#' Additionally, you may also lift directly from `11k` -> `5k`
#' (aka "double-bridge") with `bridge = "11k_to_5k"`.
#' See below for all options for the `bridge =` argument.
#'
#' Table of SomaScan Assay versions:
#' \tabular{lll}{
#' **Version** \tab **Commercial Name** \tab **Size** \cr
#' `V4` \tab 5k \tab 5284 \cr
#' `v4.1` \tab 7k \tab 7596 \cr
#' `v5.0` \tab 11k \tab 11083 \cr
#' }
#'
#' @section Extra Columns:
#' Newer versions of SomaScan typically have additional content, i.e.
#' new reagents added to the multi-plex assay that bind to new proteins.
#' Reagents that are _not_ in the "earlier" version (i.e. new reagents)
#' are maintained their value unmodified in the returned object.
#' Users may need to drop these columns in order to combine data with
#' that of a study originating with the previous SomaScan version, e.g. with
#' [collapseAdats()].
#'
#' @inheritParams params
#' @param bridge The direction of the lift (i.e. bridge).
#' @param anno.tbl Deprecated.
#' @return A "lifted" `soma_adat` object corresponding to the scaling
#' reference in the `anno.tbl`. RFU values are rounded to 1 decimal to
#' match standard SomaScan delivery format.
#' @return [lift_adat()]: A "lifted" `soma_adat` object corresponding to
#' the scaling requested in the `bridge` param. RFU values are
#' rounded to 1 decimal place to match standard SomaScan delivery format.
#' @examples
#' # `example_data` is SomaScan V4 (5k)
#' # `example_data` is SomaScan (V4, 5k)
#' adat <- head(example_data, 3L)
#' getSomaScanVersion(example_data)
#' getSomaScanVersion(adat)
#'
#' # perform the 'lifting'
#' lift_7k <- lift_adat(adat, "5k_to_7k")
#' is_lifted(lift_7k)
#' getSignalSpace(adat)
#'
#' # perform 'lift'
#' lift_11k <- lift_adat(adat, "5k_to_11k")
#' is_lifted(lift_11k)
#'
#' # attributes updated to reflect the 'lift'
#' attr(lift_7k, "Header")$HEADER$SignalSpace
#' attr(lift_7k, "Header")$HEADER$ProcessSteps
#' attr(lift_11k, "Header")$HEADER$SignalSpace
#' attr(lift_11k, "Header")$HEADER$ProcessSteps
#'
#' @importFrom tibble enframe deframe
#' @importFrom lifecycle deprecated is_present deprecate_warn
#' @export
Expand Down Expand Up @@ -100,7 +123,7 @@ lift_adat <- function(adat,
#' in the `soma_adat` attributes.
#'
#' @rdname lift_adat
#' @return Logical. Whether `adat` has been lifted.
#' @return [is_lifted()]: Logical. Whether `adat` has been lifted.
#' @export
is_lifted <- function(adat) {
x <- attr(adat, "Header.Meta")$HEADER
Expand Down
69 changes: 47 additions & 22 deletions man/lift_adat.Rd

Some generated files are not rendered by default. Learn more about how customized files appear on GitHub.

0 comments on commit 2fe5009

Please sign in to comment.