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Exporting count matrix #48
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Could you clarify what you mean by the count matrix? |
Oh sorry, I would explain it clearly. Crispulator simulates the deep sequencing, and we can have read numbers of each sgRNA for low/high reporter bin. I want to have the read numbers once I run the tool, and I called it count matrix because we can have the sgRNA read numbers for each bin with the form of n x 2 matrix (n is the number of sgRNA, and columns are for low/high bin). I hope I clarify it well. Thank you |
Sorry for not getting back to you sooner. Crispulator does generate your count matrix, but doesn't export it currently. It should be relatively easy to add. |
Hello @tlnagy, I recently realized that you provide the Thank you, |
Let me know if |
I think it is a kind of okay if I want to build single counts for each group. However, I'd like to have any idea how to build the matrix under below conditions:
Thank you, Hyun-Hwan Jeong |
Are you simulating a FACS Screen? |
Yes, for the case. |
I realized that EDIT: It sounds like you I think that would involve exporting Crispulator.jl/src/simulation/selection.jl Lines 20 to 22 in 46a804c
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Hello @tlnagy, Thank you and Best, Hyun-Hwan Jeong |
I see what you want. So I've had to rearchitect |
I appreciate your support! Kind Regards, Hyun-Hwan Jeong |
Hey @hyunhwaj, if you get a chance can you check out the notebook in #53? It has the Unsorted, GFP_low, GFP_high bins that you were looking for. Let me know over there if it works for you. You should be able to check it out using git fetch origin pull/53/head:add-multibin-support
git checkout add-multibin-support in the Crispulator directory |
I merged #53 into master so now you should be able to run Pkg.checkout("Crispulator") to give it a spin. Please open a new issue if you run into any issues or have suggestions! |
That's actually what I've wanted! I checked the notebook, and this ran without any problem. I truly appreciate your work. Hyun-Hwan Jeong |
Thanks! Let me know if you need anything else. |
Hello,
I am wondering how I can export the count matrix which is derived from the input parameters. I just only able to have the values of screening performance of the given simulated study as the output of Crispulator. Are you providing any output/function which reveals the count matrix of each sample? If it is not then could you advise me to implement the function?
Thank you,
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