Species Characteristics and Genomic Resources

Here we will list out key species (corresponding to sub-efforts), describing them briefly and provide links to canonical genomic resources to use for analysis.

TLDR: This is where we hope to have sequence files and genome feature files.

D) Acropora pulchra

E) Porites evermanni

F) Pocillopora tuahiniensis

Table denoted samples for D, E, and F

Sample Name*	Species/Strain*
POR-71-S1-TP2	Porites evermanni
POR-73-S1-TP2	Porites evermanni
POR-76-S1-TP2	Porites evermanni
POR-79-S1-TP2	Porites evermanni
POR-82-S1-TP2	Porites evermanni
POC-47-S1-TP2	Pocillopora tuahiniensis
POC-48-S1-TP2	Pocillopora tuahiniensis
POC-50-S1-TP2	Pocillopora tuahiniensis
POC-53-S1-TP2	Pocillopora tuahiniensis
POC-57-S1-TP2	Pocillopora tuahiniensis
ACR-140-S1-TP2	Acropora pulchra
ACR-145-S1-TP2	Acropora pulchra
ACR-150-S1-TP2	Acropora pulchra
ACR-173-S1-TP2	Acropora pulchra
ACR-178-S1-TP2	Acropora pulchra

sRNA-seq Sample Name	Species/Strain
ACR-140-S1-TP2	Acropora pulchra
ACR-145-S1-TP2	Acropora pulchra
ACR-150-S1-TP2	Acropora pulchra
ACR-173-S1-TP2	Acropora pulchra
ACR-178-S1-TP2	Acropora pulchra
POC-47-S1-TP2	Pocillopora tuahiniensis
POC-48-S1-TP2	Pocillopora tuahiniensis
POC-50-S1-TP2	Pocillopora tuahiniensis
POC-53-S1-TP2	Pocillopora tuahiniensis
POC-57-S1-TP2	Pocillopora tuahiniensis
POR-73-S1-TP2	Porites evermanni
POR-79-S1-TP2	Porites evermanni
POR-82-S1-TP2	Porites evermanni

Some samples failed QC and did not get sequenced for sRNA-seq.

Acropora pulchra

Characters: Colonies are arborescent with cylindrical branches but may be corymbose, with horizontally fused basal branches which have upturned ends. They may form stands more than 5 metres across. Branches are smaller than those of the large staghorn Acropora species. Radial corallites are of mixed sizes, with projecting lower lips.

Colour: Pale to dark brown or blue, often with pale blue tips.

Similar Species: May superficially resemble Acropora muricata and A. yongei, but radial corallites are distinctive. Acropora aspera has similar radial corallites but these are much larger and more scale-like. See also A. loisetteae.

Habitat: Usually restricted to shallow back-reef margins.

Abundance: Usually uncommon but may be a dominant species.

Genomic Resources

Genome

GCF_013753865.1_Amil_v2.1_genomic.fna - http://gannet.fish.washington.edu/seashell/snaps/GCF_013753865.1_Amil_v2.1_genomic.fna

md5: e4491d2710f86311e53680c5ca23c232

GCF_013753865.1_Amil_v2.1_genomic.gff - http://gannet.fish.washington.edu/seashell/snaps/GCF_013753865.1_Amil_v2.1_genomic.gff

md5: eca0bae84f91c3264287f76afbe86189

Porites evermanni

porites-evermanni

from CORALS of the World

Characters: Colonies are massive with a tendency to form columns. Tentacles are usually extended during the day.

Colour: Usually mustard, sometimes brown.

Similar Species: Readily distinguished by extended polyps giving colonies a distinctive colour and a furry appearance. Corallites are similar to those of Porites australiensis and P. columnaris.

Habitat: Shallow protected reef environments, especially lagoons.

Abundance: Usually uncommon.

Genomic Resources

Genome:

Porites_evermanni_v1.fa: https://gannet.fish.washington.edu/seashell/snaps/Porites_evermanni_v1.fa : downloaded https://www.genoscope.cns.fr/corals/genomes.html on 062923
md5: 8e58bf0417bab85a2882f95fb2e8bb56
Porites_evermanni_v1.annot.gff: https://gannet.fish.washington.edu/seashell/snaps/Porites_evermanni_v1.annot.gff : downloaded https://www.genoscope.cns.fr/corals/genomes.html on 062923
md5: 9ef993fdb4ae01ea1a6c64c855dd8472

Transcripts

Porites_evermanni_CDS.fasta - https://gannet.fish.washington.edu/kdurkin1/deep-dive/E-Peve/data/Porites_evermanni_CDS.fasta

Constructed by extracting and aligning all CDS sequences from the gff Porites_evermanni_v1.annot.gff (https://gannet.fish.washington.edu/kdurkin1/deep-dive/E-Peve/data/Porites_evermanni_v1.annot.gff, downloaded from https://www.genoscope.cns.fr/corals/genomes.html March 11, 2022) with scaffold Porites_evermanni_v1.fa (https://gannet.fish.washington.edu/kdurkin1/deep-dive/E-Peve/data/Porites_evermanni_v1.fa, downloaded from https://www.genoscope.cns.fr/corals/genomes.html March 11, 2022). Code for transcripts fasta construction here: https://github.com/urol-e5/deep-dive/blob/main/E-Peve/code/12-Peve-RNAseq-kallisto.md

sRNA

known_miRNAs.gff3 - https://gannet.fish.washington.edu/Atumefaciens/20230731-peve-E5_coral-ShortStack-sRNAseq/P_evermanni/ShortStack_out/known_miRNAs.gff3
- md5: 5df2467b645659472bc23d041756e94e
known_miRNAs.fasta - https://gannet.fish.washington.edu/Atumefaciens/20230731-peve-E5_coral-ShortStack-sRNAseq/P_evermanni/ShortStack_out/known_miRNAs.fasta
- md5: 442af6be35fa6870a88db7908aaf25c2

lncRNA

Peve_lncRNAs.fasta - https://raw.githubusercontent.com/urol-e5/deep-dive/main/E-Peve/output/Peve_lncRNA.fasta
Peve_lncRNA.bed - https://github.com/urol-e5/deep-dive/blob/main/E-Peve/output/Peve_lncRNA.bed

Proteins

Porites_evermanni_v1.annot.pep.fa: https://gannet.fish.washington.edu/seashell/snaps/Porites_evermanni_v1.annot.pep.fa : downloaded https://www.genoscope.cns.fr/corals/genomes.html on 062923
md5: 3fce204309b6bd6fc03370512f1d81a9

Pocillopora tuahiniensis

Screen Shot 2024-02-06 at 10 46 45 AM

Johnston et al 2023

Characters: The corallum is hemispherical and the branches are fairly evenly spaced, separated by ap- proximately 1–2 cm. Branches are straight and robust, and most branches ramify. Verrucae are evenly distributed and are equal in size and shape, approximately 1 mm. Verrucae are not verrucose at their tips, but rather are jagged and crown shaped.

Colour: Colonies may be brown, pink, or pale yellow, and like many Pocil- lopora species, have pigment concentrated in the oral opening of the polyps, forming a dark ring. .

Sister Species: Pocillopora verrucosa, which has relatively prominent verrucae.

Habitat: Shallow reef environments to deep forereef.

Abundance: Common on lagoon and forereef.

Genomic Resources from other Pocillopora species

Genome

https://owl.fish.washington.edu/halfshell/genomic-databank/Pocillopora_meandrina_HIv1.assembly.fasta
- Downloaded 20230519: http://cyanophora.rutgers.edu/Pocillopora_meandrina/

Genes

Pocillopora_meandrina_HIv1.genes.gff3 (55MB)
- MD5 checksum: ace5c9a588321fada8e6771a1c758861
- Downloaded 20230519: http://cyanophora.rutgers.edu/Pocillopora_meandrina/
- NOTE: This is labelled as a GFF3, but in reality is a GTF file; as it only contains transcript/exon/CDS features. There are no gene features (e.g. 5'/3'UTR, mRNA, etc)