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kymographs

Automated kymographs from MOV files

  1. Download 'script.sh' and make it executable by command

chmod +x extract.sh

  1. Place your MOV movie files in selected directory

  2. If you are working on computer cluster start a job by (-I is with uppercase i)

qsub -I

  1. On computer cluster, software module must be loaded

module load ffmpeg

  1. run script 'extract.sh' with MOV directory parameter, e.g.

if script is in the actual directory

./extract.sh /home/me/Movies

or if the srcipt is anywhere

/home/me/scripts/extract.sh /home/me/Movies

This script generates a set of directories with individual frames from movies. These shots are taken from several 2-second time points spaced at randomly selected intervals between 2 and 30 seconds.

Directories are named <movie_name>-<time_poin>

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Automated kymographs from MOV files

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