- Supports Python 3.5+
- Drops support for Python 3.4
- Fix issue: "seqmagick with no params gives KeyError:None" [GH-77]
- Fix for Biopython 1.71 dual coding support [GH-76]; also fixes issue: "Translation error with new BioPython" [GH-79]
- Send logging to stderr, not stdout [GH-75]
- Supports Python 3.4+
- Drops support for python 2.7
- requires biopython >= 1.70
- Drops support for bz2 compression [see GH-66]
- New option
convert --sample-seed
to make--sample
deterministic.
- New
quality-filter --pct-ambiguous
switch [GH-53] - setup.py enforces biopython>=1.58,<=1.66 (1.67 is not compatible) [GH-59]
- This is the last release that will support Python 2!
- Allow string wrapping when input isn't FASTA. [GH-45]
- Fix
--pattern-include
,--pattern-exclude
, and--pattern-replace
for sequences without descriptions (e.g., from NEXUS files). [GH-47] - Fix mogrify example. [GH-52]
- Map
.nex
extension to NEXUS-format (--alphabet must be specified if writing) - Use reservoir sampling in
--sample
selector (lower memory use) - Support specifying negative indices to
--cut
[GH-33] - Optionally allow invalid codons in
backtrans-align
[GH-34] - Map
.fq
extension to FASTQ format - Optional multithreaded I/O in
info
[GH-36] - Print sequence name on length mismatch in
backtrans-align
[GH-37] - Support for
+
and-
in head and tail to mimick Linux head and tail commands. - Fix scoring for mixed-case sequences in
primer-trim
. - Fix bug in
primer-trim
- failed when sequence had multiple 5' gaps compared to the primer. - Clarify documentation and fix bug in convert/mogrify
--pattern-replace
[GH-39] - Support for gzip files in
seqmagick convert --sort
- Change
seqmagick extract-ids --source-format
to--input-format
to match other commands (GH-29) - Support gzip- and bzip2-compressed inputs and outputs for most commands (GH-30)
- Change default input format for
sff
tosff-trim
, which respects the clipping locations embedded in each sequence record. - Add
--details-out
option toseqmagick quality-filter
, which writes details on each read processed. - Match barcode/primer
seqmagick quality-filter
against a trie; allows per-specimen barcodes. - Remove
--failure-out
option fromseqmagick quality-filter
. See--details-out
- Raise an error if number of codons does not match number of amino acids in
seqmagick backtrans-align
- Add
--sample
subcommand (GH-31)
- Fix bug in
--squeeze
- More informative messages in
seqmagick primer-trim
- Added
--alphabet
flag to allow writing NEXUS (GH-23) - Exiting without error on SIGPIPE in extract-ids, info (GH-17)
- Ambiguities are translated as 'X' in --translate (GH-16)
- Allowing '.' or '-' as gap character (GH-18)
--name-prefix
and--name-suffix
no longer create a mangled description (GH-19)- Files owned by another user can be mogrified, as long as they are group writeable (GH-14)
- Add
backtrans-align
subcommand, which maps unaligned nucleotides onto a protein alignment (GH-20) - Allow FASTQ as input to quality-filter
- Significantly expand functionality of quality-filter: identify and trim barcodes/primers; report detailed failure information.
- Cleanup, additional tests
- Add
--drop
filter to convert and mogrify (GH-24) - Apply current umask when creating files (GH-26)
- Support stdin in
seqmagick info
(GH-27) - Support translating ambiguous nucleotides, if codon translation is unambiguous
- Fix bug in
quality-filter
MinLengthFilter - Case consistency in seqmagick
- Internal reorganization - transformations are converted to partial functions, then applied.
- Argument order now affects order of tranformation application.
- Change default output format to 'align' for TTYs in seqmagick info
- Add BioPython as dependency (closes GH-7)
- Add
primer-trim
subcommand - Add option to apply custom function(s) to sequences
- Add new filtering options:
--squeeze-threshold
,--min-ungapped-length
--include-from-file
--exclude-from-file
- Removed seqmagick muscle
- Added new subcommand
quality-filter
- Added new subcommand
extract-ids
(closes GH-13) - Allow use of '-' to indicate stdin / stdout (closes GH-11)
- Add mapping from .phyx to
phylip-relaxed
(targeted for BioPython 1.58)
- Refactoring
- Added hyphenation to multi-word command line options (e.g.
--deduplicatetaxa
->--deduplicate-taxa
) - Add support for
.needle
,.sff
formats - Close GH-4
Initial release