error:Can't find snv in ss!i

[Angelven]

Hi Qing.

I made a input.vcf containing about 660000 SNVs and about 15% were in dbSNP.

I got an error while running giremi:

             [mpileup] 1 samples in 1 input files
             error:Can't find snv in ss!i

Does it mean snv was not found in .bam file?

Also, I made a small input.vcf from the large vcf, it contains about 20000 SNVs. GIREMI seems to run well.

Best.
Li

[Angelven]

To find the error snv, I made a smaller input.vcf (3301 snvs) and run "strace ./giremi", error occures again:

 munmap(0x2ac0bca65000, 2289664)         = 0
 munmap(0x2ac0bcc94000, 1146880)         = 0
 munmap(0x2ac0bb97c000, 17731584)        = 0
 close(4)                                = 0
 munmap(0x2ac0bcdac000, 135536640)       = 0
 fstat(1, {st_mode=S_IFREG|0600, st_size=251, ...}) = 0
 mmap(NULL, 4096, PROT_READ|PROT_WRITE, MAP_PRIVATE|MAP_ANONYMOUS, -1, 0) = 0x2ac0bb97c000
 write(1, "coor:235473079\tchr:chr1coor:2354"..., 4096) = 4096
 write(2, "error:Can't find snv in ss!i\n", 29error:Can't find snv in ss!i

) = 29
--- SIGSEGV (Segmentation fault) @ 0 (0) ---
+++ killed by SIGSEGV (core dumped) +++

So, I thought snv chr1:235473079 maybe the troublemaker, then I only extracted 1500 snvs (contain snv chr1:235473079 and snvs surrounding it) from the 3301 list, it runs well:

 munmap(0x2b9a071a7000, 4096)            = 0
 write(1, "43258455\tchr:chr1coor:243258455\t"..., 4096) = 4096
 rt_sigaction(SIGINT, {SIG_IGN, [], SA_RESTORER, 0x30894326a0}, {SIG_DFL, [], 0}, 8) = 0
 rt_sigaction(SIGQUIT, {SIG_IGN, [], SA_RESTORER, 0x30894326a0}, {SIG_DFL, [], 0}, 8) = 0
 rt_sigprocmask(SIG_BLOCK, [CHLD], [], 8) = 0
 clone(child_stack=0, flags=CLONE_PARENT_SETTID|SIGCHLD, parent_tidptr=0x7fff29ce8078) = 29120
 wait4(29120, [{WIFEXITED(s) && WEXITSTATUS(s) == 0}], 0, NULL) = 29120
 rt_sigaction(SIGINT, {SIG_DFL, [], SA_RESTORER, 0x30894326a0}, NULL, 8) = 0
 rt_sigaction(SIGQUIT, {SIG_DFL, [], SA_RESTORER, 0x30894326a0}, NULL, 8) = 0
 rt_sigprocmask(SIG_SETMASK, [], NULL, 8) = 0
 --- SIGCHLD (Child exited) @ 0 (0) ---
 write(2, "Analysis DONE!\n", 15Analysis DONE!
)        = 15
 write(1, "ore '--args finput=\"./GoldenSet/"..., 239) = 239
 exit_group(1)                           = ?

And I also got the .res file. It seems giremi can find this snv in .bam.
So I wonder how this error occure and what should I do to fix it.

[zhqingit]

Hi Li,

Based on my experience, the error is caused by the bam file in which some
reads map to more than one chromosomes. I think you can try test this point.

Best,
Qing

2016-03-20 20:03 GMT-07:00 Angelven notifications@github.com:

To find the error snv, I made a smaller input.vcf (3301 snvs) and run
"strace ./giremi", error occures again:

munmap(0x2ac0bca65000, 2289664) = 0
munmap(0x2ac0bcc94000, 1146880) = 0
munmap(0x2ac0bb97c000, 17731584) = 0
close(4) = 0
munmap(0x2ac0bcdac000, 135536640) = 0
fstat(1, {st_mode=S_IFREG|0600, st_size=251, ...}) = 0
mmap(NULL, 4096, PROT_READ|PROT_WRITE, MAP_PRIVATE|MAP_ANONYMOUS, -1, 0) = 0x2ac0bb97c000
write(1, "coor:235473079\tchr:chr1coor:2354"..., 4096) = 4096
write(2, "error:Can't find snv in ss!i\n", 29error:Can't find snv in ss!i

) = 29
--- SIGSEGV (Segmentation fault) @ 0 (0) ---
+++ killed by SIGSEGV (core dumped) +++

So, I thought snv chr1:235473079 maybe the troublemaker, then I only
extracted 1500 snvs (contain snv chr1:235473079 and snvs surrounding it)
from the 3301 list, it runs well:

munmap(0x2b9a071a7000, 4096) = 0
write(1, "43258455\tchr:chr1coor:243258455\t"..., 4096) = 4096
rt_sigaction(SIGINT, {SIG_IGN, [], SA_RESTORER, 0x30894326a0}, {SIG_DFL, [], 0}, 8) = 0
rt_sigaction(SIGQUIT, {SIG_IGN, [], SA_RESTORER, 0x30894326a0}, {SIG_DFL, [], 0}, 8) = 0
rt_sigprocmask(SIG_BLOCK, [CHLD], [], 8) = 0
clone(child_stack=0, flags=CLONE_PARENT_SETTID|SIGCHLD, parent_tidptr=0x7fff29ce8078) = 29120
wait4(29120, [{WIFEXITED(s) && WEXITSTATUS(s) == 0}], 0, NULL) = 29120
rt_sigaction(SIGINT, {SIG_DFL, [], SA_RESTORER, 0x30894326a0}, NULL, 8) = 0
rt_sigaction(SIGQUIT, {SIG_DFL, [], SA_RESTORER, 0x30894326a0}, NULL, 8) = 0
rt_sigprocmask(SIG_SETMASK, [], NULL, 8) = 0
--- SIGCHLD (Child exited) @ 0 (0) ---
write(2, "Analysis DONE!\n", 15Analysis DONE!
) = 15
write(1, "ore '--args finput="./GoldenSet/"..., 239) = 239
exit_group(1) = ?

And I also got the .res file. It seems giremi can find this snv in .bam.
So I wonder how this error occure and what should I do to fix it.

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#9 (comment)

[Angelven]

Hi Qing,

You get the right point. I split my input.vcf into 24 chromosomes, and each of them runs well.

Because giremi will calculate MI first from the total set of snvs and it is improper to split my input, so I will re-filter my input.bam file.

Thank you.

Best,
Li