Dale's 3 day imagej course slides and exercises
It is a mixture of lectures, demos, hands on sessions and exercises.
Day 1 Understanding digital images. Bit depth, brightness, colours & pixels. ImageJ / Fiji basics, getting to grips with the software. Image presentation (generating figures for publication). ROIs and simple measurements.
Day 2 Image analysis. Pre-processing (Filters, background), thresholding for feature identification and counting. Alterntaive segmentation strategies. 2D 3D and timeseries. Identifying double / triple stained cells, (and why this isn't colocalisation).
Day 3 ImageJ / Fiji macros. Recording and writing custom scripts to automate image analysis.