CASQITO

Computer Assisted Signal Quantification Including Threshold Options

This Fiji macro is under GPL license. This macro workflow is detailed in "Apoptosis quantification in tissue: development of a semi automatic protocol and assessment of critical steps of image processing"

Prerequisites:

CASQITO runs on Fiji and only treat .lif files of stacks images.

Install & Launch:

1. Download CASQITO macro
2. Start Fiji
3. On Fiji, follow "Plugins" > "Macro" > "Run" find and select "CASQITO.ijm" file. Alternatively, drag and drop "CASQITO.ijm" file on Fiji and click "run".
4. Follow the instructions step by step.

Outputs:

CASQITO can output up to 4 types of files

. A .xls table containing threshold values

. A .png picture displaying a histogram of the threshold values

. A .xls table containing quantification results

. Several .png pictures displaying ROIs determined for zone selection

Citation:

Juliette de Noiron, Marion Hoareau, Jessie Colin, Isabelle Guénal. Apoptosis quantification in tissue: development of a semi automatic protocol and assessment of critical steps of image processing. 2021. hal-03297234