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Localized reconstruction of flexible subunits from cryo-EM images
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relion_localized_reconstruction.py

README.md

Note that this version of the software is now deprecated. The latest version of Localized Reconstrution is part of Scipion 2.0. The development version of the code is here: https://github.com/scipion-em/scipion-em-localrec

Localized Reconstruction

Localized reconstruction of flexible subunits from macromolecular complexes.

Version: 1.2.1

It also requires the following software:

  • Scipion 1.1 (or later - notice that a patch is required to use the current version in Scipion)
  • Relion 1.4

Reference

If localized reconstruction is useful in your work, please cite:

Ilca SL, Kotecha A, Sun X, Poranen MM, Stuart DI & Huiskonen JT (2015). Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes. Nat Commun 6, 8843. doi:10.1038/ncomms9843

Instructions

See: https://github.com/OPIC-Oxford/localrec/wiki

Acknowledgements

The authors acknowledge funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (649053) and from the Wellcome Trust PhD Programme in Structural Biology.

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