APIs for the Sader Method
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README.md

README.md

APIs for the Sader Method GCI

https://sadermethod.org/

We have developed an API interface that lets you seamlessly use the Global Calibration Initiative directly on your AFM. This development is free to all AFM users.

At the moment we have a version compatible with Asylum Research systems and with this API interface, you can effortlessly upload data to the GCI and access its worldwide database - while using the standard Thermal Tune of the Asylum AFM.

We plan on adding support for all AFM versions in the near future with your help!

Please email feedback (quoting version number and AFM type): support@sadermethod.org

We hope that this new development helps to facilitate the worldwide standardisation of AFM force measurements.

Using the API Interface for Asylum Research

Enter your username and password for the Sader Method GCI (sadermethod.org), and press Enter, to enable the GCI Panel.

Initial Interface

Thermal frequency, quality factor and spring constant in the GCI Panel are taken directly from the Thermal Panel in the Asylum Research AFM Software.

Retract your cantilever at least 100 μm from the surface prior to taking a Thermal.

##Approach 1:

  • First calibrate the spring constant using the Thermal Method.
  • Tick the Enable checkbox next to Thermal Spring Constant.
  • Select your Lever from the pull down menu and press ‘Upload & Calculate’.
  • The frequency, quality factor and spring constant are merged with the GCI database. The Sader Method gives a new calculation of your spring constant and its uncertainty.

Initial Interface

##Approach 2:

  • Measure and fit a Thermal only (InvOLS not required).
  • Ensure the Enable checkbox next to Thermal Spring Constant is NOT ticked.
  • Select your Lever from the pull down menu and press ‘Calculate’.
  • Only the frequency and quality factor are sent to the GCI. The Sader Method uses the existing database to calculate your spring constant and its uncertainty.

Initial Interface

Approach 1 lets users contribute their data to the international database, and compares their calibration measurements to those of others - letting users help each other and providing a global reference point for AFM force measurements.

The Thermal spring constant is your locally calibrated value, whereas the Sader spring constant is standardised internationally. Check your measurements if there is a large difference between these values; see Reference (below).

  • Request a lever: Users request cantilever models to be added to the GCI via this link.
  • Register: Users register via this link.
  • Status: Indicates if InvOLS is calibrated and whether Approach 1 or 2 is used.

NOTE: Take measurements of the fundamental flexural mode in air. The GCI reports the static normal spring constant at the imaging tip position.

Reference: Sader et al., Review of Scientific Instruments, 87, 093711 (2016).