@EvoleneP The NI-based galvo that performs the z-stack is failing when the triggered sine-wave generation on the shadow reduction galvo is enabled. Error thrown is NI status code -200963. Timing type of the galvo is incompatible. <img width="1442" alt="image" src="https://github.com/user-attachments/assets/44bb874d-ebcb-4131-b78b-df28c76a1c3c">