This repository contains all documentation about theoric biological issues such as culture methods and techniques, justification, ecology and current functions of chosen microorganisms. The following one is the index kept in these documents.
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Introduction to cellular colonies
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Morphological classification of colonies
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Importance of morphological analysis
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Bibliography
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Lactobacillus casei
1.1. Object of study and interest of L.casei
1.2. In vitro culture of L.casei
1.3. Ecology and growth of L.casei
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Saccharomyces cerevisiae
2.1. Object of study and interest of S. cerevisiae
2.2. In vitro culture of S. cerevisiae
2.3. Ecology and growth of S. cerevisiae
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Bibliography
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What does Tolerance Limits mean?
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Classification of tolerance limits.
2.1. Temperature
2.2. Salinity
2.3. Humidity
2.4. Radiation
2.5. pH
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Antibiotics as a limiting factor.
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Bibliography
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Preparation of culture medium
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Lactobacillus casei
2.1. Culturing materials for L. casei
2.2. Procedure
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Saccharomyces cerevisiae
3.1. Culturing materials for de S. cerevisiae
3.2. Procedure
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Laboratory equipment
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Necessary materials
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Availability
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Introduction to counting methods
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Non-automatic counting techniques
2.1. Plate count
2.2. Counting by filtration
2.3. Most probable number method (NMP)
2.4. Direct counting through a microscope
2.5. Turbidity method
2.6. Determination of dry weight
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Automatic counting techniques
3.1. Counting by electrical resistance
3.2. Counting by flow cytometry
3.3. Counting by image analysis
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Bibliography