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Known problems
Sometimes it may seem that the program does all the operations in the solid space of the images (white), not the void space (black). It happens when the gray scale in tiff images is inverted. The program does not handle inverted gray scales. One way to check grayscale inversion is to open an image in ImageJ, go to Image->Show Info, look at "Bits per pixel". It will be either "8 (grayscale LUT)" (normal scale, everything's fine; LUT stands for the lookup table) or "8 (inverting grayscale LUT)" (not handled by the program).
One way to remove the inversion and to preserve the original structure is to use ImageJ. In ImageJ, go to Image->Lookup tables->Invert LUT. Now black and white colors are inverted. To return to the same picture, go to Edit->Invert.