All in R DamID sequencing pipeline (use genes as bin)
Includes adapter removal, Mapping and enrichment finding for DamID-Seq reads. This version includes gene level bins.
For more information see also: Tools for DNA adenine methyltransferase identification analysis of nuclear organization during C. elegans development
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Install R (only 64 bit version!)
R Project -
Download GeneDamIDseq_0.1.4.tar.gz
-
install additional packages needed for the pipeline in R
install.packages(c('corrplot', 'aqfig'))
source("https://bioconductor.org/biocLite.R")
biocLite()
biocLite(c("QuasR", "ShortRead", "GenomicRanges", "S4Vectors", "IRanges",
"BiocInstaller", "Biobase", "Biostrings", "BSgenome", "GenomicFeatures",
"GenomicAlignments", "BiocParallel", "GenomeInfoDb", "rtracklayer",
"GenomicFiles", "Rbowtie", "biomaRt"))
- install GeneDamIDSeq in R
(exchangePATH
with the path to the installation file on your disk)
install.packages("PATH/GeneDamIDseq_0.1.4.tar.gz", repos = NULL, type = "source")