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Eugene Katrukha edited this page May 3, 2024 · 7 revisions

Welcome to the UnequalTiffs wiki!

This is FIJI plugin for working with a set of .tif images of different sizes, but with the same number of dimensions.

The plugin can perform their concatenation to a hyperstack, making 3D/4D/5D montage, or browse them in BigDataViewer/BigVolumeViewer.

The plugin uses caching to access tif files, so it is possible to work
with a large number of images using relatively little memory.

The detailed documentation is still in development.

Installation

To install the plugin, download the latest jar file from Releases and copy it to the jars folder of your FIJI.
After restarting FIJI, it should appear in the Plugins > UnequalTiffs X.X.X menu.

If you want to use Explore BVV functionality, you have to download and unzip to the jars folder the latest release of bvv-playground.

Main functions

In all cases, the input for the plugin is a user-specified folder containing multiple tif files.
They should have the same number of dimensions, but the size of each dimension can vary (apart from the number of channels).
In addition, they all should have the same bit depth.

The output is always sorted alphabetically by filename.

There are always two options to position each tif file in the final image:
zero (top-left) or centered.

UT Montage

Creates a virtual cached montage image with a specified number of rows and columns.

UT Concatenate

Creates a virtual concatenated hyperstack image, concatenating them along a specified axis.

UT Explore BDV

If tif files are 3D, the plugin creates a 3D montage volume and loads it to BDV.
The whole montage volume can be rotated using a regular BDV mouse navigation means.
Individual volumes can be rotated by pressing and holding D button on the keyboard and moving the mouse.
Shortcut 1 centers montage volume in the center of the viewer along the global XY plane.
Shortcut 2 aligns all individual volumes to XY plane of the montage volume.
Shortcut 3 aligns all individual volumes to XZ plane (of the montage volume).
Shortcut 4 aligns all individual volumes to YZ plane (of the montage volume).
See the movie below for the illustration.

UT Explore BVV

Same as above function for 3D volumes, only using BVV (BigVolumeViewer).
At the moment, due to some missing functionality, the current version of BVV
does not allow to add more than 4-5 volumes/tiffs.
It should be fixed relatively soon.
UT_small

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