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Welcome to the MotiQ wiki! In this Wiki, we provide additional guidelines to setup your optimal MotiQ analysis. Find below links to guides for individual topics.
Please note that the MotiQ wiki is a lively platform and more content and functions of the wiki will be realized in the future!
- Step-by-step guide to find good MotiQ Thresholder settings
- Step-by-step guide to find good MotiQ 3D Analyzer settings
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MotiQ 3D Analyzer reports
No 3D visualization generated
.... What to do now? Answers and explanations provided here.
- Can I manually edit a segmented image output by MotiQ Thresholder? Yes! And, when working with cropped cells (using MotiQ Cropper), it can be more time efficient to manually remove a falsely-detected particle from a segmented image than to repeat cropping and segmentation. Explanations provided here.
- Can I use the ROIs logged by MotiQ Cropper to repeat the cropping (eventually with slight modifications)? Yes! Explanations provided here.
- Can I embed MotiQ plugins into an ImageJ macro, e.g., to combine the plugins with other ImageJ/FIJI tools or functions or streamline my analysis into one analysis pipeline? Yes! Explanations provided here.
You are looking for the answer for your question and can't find the answer here?
Don't mind reaching out via email (jan.hansen (at) uni-bonn.de) and suggest the question to be answered in the MotiQ wiki.
Copyright (C) 2017-2024: Jan N. Hansen.
MotiQ is part of the following publication: Hansen et al., MotiQ: an open-source toolbox to quantify the cell motility and morphology of microglia, Molecular biology of the cell, 33:11 (2022). https://doi.org/10.1091/mbc.E21-11-0585