Could not allocate enough memory for 90576558080 sequences

[Antony-1996]

Dear author
I encountered a problem when I use seqtk to downsample fastq file ，the result look like this：

Is there any way that I can do to solve this ? thank you so much

[tseemann]

Please use the two-pass mode: seqtk sample -2

Usage:   seqtk sample [-2] [-s seed=11] <in.fa> <frac>|<number>

Options: -s INT       RNG seed [11]
         -2           2-pass mode: twice as slow but with much reduced memory

If that works, please close this issue. Thanks!

[fidibidi]

This helped me thanks!