Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

k-mer size support >32? #3

Open
EvdH0 opened this issue Feb 22, 2019 · 0 comments
Open

k-mer size support >32? #3

EvdH0 opened this issue Feb 22, 2019 · 0 comments

Comments

@EvdH0
Copy link

EvdH0 commented Feb 22, 2019

Hi Brian,

Thanks for the rewrite! Is there a way to run meryl with a k-mer size larger than 32? Because running with the following example meryl count and meryl print

>test
ATGAAAATCAAAACTCGCTTCGCGCCAAGCCCAACAGGCTATCTGCA

with k=32 results in :

AAAACTCGCTTCGCGCCAAGCCCAACAGGCTA	1
AAAATCAAAACTCGCTTCGCGCCAAGCCCAAC	1
AAACTCGCTTCGCGCCAAGCCCAACAGGCTAT	1
AAATCAAAACTCGCTTCGCGCCAAGCCCAACA	1
AACTCGCTTCGCGCCAAGCCCAACAGGCTATC	1
AATCAAAACTCGCTTCGCGCCAAGCCCAACAG	1
ACTCGCTTCGCGCCAAGCCCAACAGGCTATCT	1
ATCAAAACTCGCTTCGCGCCAAGCCCAACAGG	1
ATGAAAATCAAAACTCGCTTCGCGCCAAGCCC	1
AGCCTGTTGGGCTTGGCGCGAAGCGAGTTTTG	1
CAGATAGCCTGTTGGGCTTGGCGCGAAGCGAG	1
CGCTTCGCGCCAAGCCCAACAGGCTATCTGCA	1
TCAAAACTCGCTTCGCGCCAAGCCCAACAGGC	1
TCGCTTCGCGCCAAGCCCAACAGGCTATCTGC	1
TTGGGCTTGGCGCGAAGCGAGTTTTGATTTTC	1
TGAAAATCAAAACTCGCTTCGCGCCAAGCCCA	1

but with k=33 results in:

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC	7

which does not seem right.

The full commands are here

meryl count  k=32 test.fna output test.k32.out.merylCount &> test.k32.out.merylCount.std
meryl count  k=33 test.fna output test.k33.out.merylCount &> test.k33.out.merylCount.std

meryl -VV print test.k32.out.merylCount > test.k32.out.histogram 2> test.k32.out.histogram.std
meryl -VV print test.k33.out.merylCount > test.k33.out.histogram 2> test.k33.out.histogram.std

and attached a tarball with the stderr etc. kmertest.tar.gz

Thanks!

Eric

brianwalenz added a commit that referenced this issue Mar 5, 2020
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant