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The --skip_fastq_download flag implies that only the fastq files will not be downloaded. When using the --skip_fastq_download and --nf_core_pipeline rnaseq together, the fastq files are not downloaded (expected), but sample sheet is not created (unexpected).
Command used and terminal output
nextflow run nf-core/fetchngs -profile docker --nf_core_pipeline rnaseq --outdir test --input temp.txt --max_cpus 2 --max_memory 5.GB --skip_fastq_download
drpatelh
changed the title
samplesheet is not created when --skip_fastq_download is given
Final samplesheet is not created when --skip_fastq_download is provided
Nov 7, 2022
drpatelh
added a commit
to drpatelh/nf-core-fetchngs
that referenced
this issue
Nov 7, 2022
A merged samplesheet will now also be generated if using --skip_fastq_download. The only difference will be that the original ftp download links will be populated in the fastq_1 and fastq_2 fields rather than the local paths relative to --outdir where fastq_1 and fastq_2 would have been downloaded if not using --skip_fastq_download. Note, that if the ftp download links don't exist as part of the ENA metadata then the fastq_1 and fastq_2 fields will be empty in the samplesheet but there is nothing we can do about that really.
Description of the bug
The
--skip_fastq_download
flag implies that only thefastq
files will not be downloaded. When using the--skip_fastq_download
and--nf_core_pipeline rnaseq
together, thefastq
files are not downloaded (expected), but sample sheet is not created (unexpected).Command used and terminal output
nextflow run nf-core/fetchngs -profile docker --nf_core_pipeline rnaseq --outdir test --input temp.txt --max_cpus 2 --max_memory 5.GB --skip_fastq_download
Relevant files
temp.txt
System information
N E X T F L O W ~ version 22.04.5
Launching
https://github.com/nf-core/fetchngs
[nostalgic_meitner] DSL2 - revision: b79cde2 [master]The text was updated successfully, but these errors were encountered: