Streamlined DAM Analysis

DAM: Drosophila Activity Monitor

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Please refer to the README and INSTRUCTIONS documents in the blips and streamline2.0 directories for more information on each "package"

Packages

• blips: Process blips generated by DAM monitors and fix the blips pre-ShinyR DAM
• streamline2.0: Process post-ShinyR DAM output data for streamline analysis

Steps

1. Download a zip file of the repository to your working directory on Secretariat

    cd <project_directory_path>
    wget 'https://github.com/ypan23-1876660/DAM/archive/refs/heads/main.zip'
    unzip main.zip && rm main.zip
   

   Make sure you are logged into the head / login node (secretariat-master) for this step!

2. Edit the variables in blips_shscript.sh in blips/scripts

3. Run the blips initiator script:

    cd <project_directory_path>/blips/scripts
    sbatch blips_initiator.sh
   

4. Download the results from (2) and run ShinyR DAM

5. Upload the results from (3) to Secretariat

6. Edit the variables in streamline_shscript2.0.sh in streamline2.0/scripts

7. Run the streamline2.0 initiator script:

    cd <project_directory_path>/streamline2.0/scripts
    sbatch streamline_initiator2.0.sh
   

8. Download the results for further analysis (e.g. SAS)