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Honeychrome v0.8.2
Honeychrome v0.8.2 Release Notes
- Added support for FCS files for the following spectral cytometers, so that metadata, channels and ranges are properly processed, allowing for the peculiarities of FCS files from the various manufacturers:
** Cytek Aurora & Northern Lights
** Sony ID7000
** BD FACSDiscover A8 & S8
** BD FACSymphony A5SE
** Beckman Coulter CytoFLEX mosaic
** ThermoFisher Attune Xenith
** NovoCyte Opteon
Additionally the previous v0.8 Release Notes:
This release contains several new features, improvements and bug fixes.
Major new features
- The two major components of AutoSpectral have been added: Control Cleaning and Autofluorescence. These (optional) enhancements of the unmixing workflow are rapidly becoming the standard tool for fixing unmixing artifacts caused by variable autofluorescence.
- tabbed plugin system: Honeychrome now automatically loads plugins from a plugins folder; two example plugins included. Note that plugins are currently only available if Honeychrome is run in the Python environment (see installation instructions), not as a precompiled executable.
- 2D plots can now be shown as dot plots coloured by gate
- debug data: Honeychrome can send error/crash data if allowed by the user. Note that no personal identifiable information is collected or sent.
PyPi release
- You can now use Honeychrome in a Python environment, simply by issuing the command: "pip install honeychrome", then run "honeychrome" from the command line
Numerous improvements, including the following
- automatic recognition of antigens and fluorophore names in the spectral model editor
- better colourmap ranges to avoid saturation
- imported FCS files can now live anywhere: if they are not in the experiment folder, a symbolic link is set up from the experiment folder, so no more broken relative paths
- build system now produces executable installers for Linux (Ubuntu > 24.04), Windows (10, 11), and MacOs (ARM). Hopefully this will now cover (almost) everyone who had trouble running the previous executables
Also numerous bug fixes.