Skip to content

Honeychrome v0.8.2

Choose a tag to compare

@salmansamson salmansamson released this 01 Jun 12:32
· 503 commits to main since this release

Honeychrome v0.8.2 Release Notes

  • Added support for FCS files for the following spectral cytometers, so that metadata, channels and ranges are properly processed, allowing for the peculiarities of FCS files from the various manufacturers:
    ** Cytek Aurora & Northern Lights
    ** Sony ID7000
    ** BD FACSDiscover A8 & S8
    ** BD FACSymphony A5SE
    ** Beckman Coulter CytoFLEX mosaic
    ** ThermoFisher Attune Xenith
    ** NovoCyte Opteon

Additionally the previous v0.8 Release Notes:

This release contains several new features, improvements and bug fixes.

Major new features

  • The two major components of AutoSpectral have been added: Control Cleaning and Autofluorescence. These (optional) enhancements of the unmixing workflow are rapidly becoming the standard tool for fixing unmixing artifacts caused by variable autofluorescence.
  • tabbed plugin system: Honeychrome now automatically loads plugins from a plugins folder; two example plugins included. Note that plugins are currently only available if Honeychrome is run in the Python environment (see installation instructions), not as a precompiled executable.
  • 2D plots can now be shown as dot plots coloured by gate
  • debug data: Honeychrome can send error/crash data if allowed by the user. Note that no personal identifiable information is collected or sent.

PyPi release

  • You can now use Honeychrome in a Python environment, simply by issuing the command: "pip install honeychrome", then run "honeychrome" from the command line

Numerous improvements, including the following

  • automatic recognition of antigens and fluorophore names in the spectral model editor
  • better colourmap ranges to avoid saturation
  • imported FCS files can now live anywhere: if they are not in the experiment folder, a symbolic link is set up from the experiment folder, so no more broken relative paths
  • build system now produces executable installers for Linux (Ubuntu > 24.04), Windows (10, 11), and MacOs (ARM). Hopefully this will now cover (almost) everyone who had trouble running the previous executables

Also numerous bug fixes.