Installation

External programs

To enjoy the full functionaly of TranSPHIRE, install the following programs:

IMOD 4.9: http://bio3d.colorado.edu/imod
MotionCor2 1.0.0/1.0.5/1.1.0: http://msg.ucsf.edu/em/software/motioncor2.html
SumMovie 1.0.2: http://grigoriefflab.janelia.org/unblur
CTER 1.0: http://sphire.mpg.de
CTFFIND 4.1.8/4.1.10: http://grigoriefflab.janelia.org/ctf
Gctf 1.06,1.18: https://www.mrc-lmb.cam.ac.uk/kzhang
crYOLO 1.0.4/1.0.5/1.1.0: http://sphire.mpg.de

Installation

TranSPHIRE needs an environment that includes Python version 3.6, QT version 5 and PyQt version 5.

Installation using Anaconda

https://www.anaconda.com/download/#linux

Create a new virtual environment

conda create -n transphire python=3.6 pyqt=5

Activate the environment

source activate transphire

or

conda activate transphire

Depending on your installation of anaconda.

Install transphire

pip install transphire

Installation inside your own environment that matches the requirements (Not recommended)

Install transphire

pip install transphire

Ready to configure TranSPHIRE

You are now ready to configure your TranSPHIRE Installation