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A tool for fast automatic 3D-stitching of teravoxel-sized microscopy images (BMC Bioinformatics 2012, 13:316)
TeraStitcher is a free tool that enables the stitching of Teravoxel-sized tiled microscopy images even on workstations with relatively limited resources of memory (<8 GB) and processing power. It exploits the knowledge of approximate tile positions and uses ad-hoc strategies and algorithms designed for such very large datasets. The produced images can be saved into a multiresolution representation to be efficiently visualized (see Vaa3D-TeraFly) and processed.
Images for this research were produced by the Biophisics lab of LENS. The software was developed by the Integrated Research Centre of University Campus Bio-Medico of Rome (Italy).
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Download and install |
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User's guide |
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Developer's guide |
- designed for images exceeding the TeraByte size
- fast and reliable 3D stitching based on a multi-MIP approach
- typical memory requirement below 4 GigaBytes (8 at most)
- 2D stitching also supported (experimental)
- support for image files bigger than 2 GB
- regular expression based matching for image file names
- data subset selection
- sparse data support
- i/o plugin-based architecture
- stop and automatic resume of the last step of stitching (merge)
--libtiff_uncompress and --libtiff_rowsperstrip and fixes a bug in the GUI preventing pure 2D stitching. See the latest binary release!
IMG_REGEX xml attribute and download the latest release!.
Post-doctoral Fellow at University of Cassino and L.M. (Italy)
Main developer
Full Professor at University Campus Bio-Medico of Rome (Italy)
Supervisor and co-developer
Post-doctoral Fellow at University Campus Bio-Medico of Rome (Italy)
Co-developer