Step 4: Threshold

In this step, TeraStitcher evaluates the displacements reliability according to a given threshold between 0(unreliable) and 1(reliable).

If reliability is lower than the given threshold, the displacement is substituted with a new one which uses nominal stages coordinates. If a tile does not have any reliable displacements with its neighbors, it is marked as a nonstitchable tile. Nonstitchable tiles can be optionally excluded from stitching in the Merge step.

Prerequisites

• an XML descriptor generated by the Project step or, alternatively, an XML descriptor in which one (and only one) displacement is stored for each pair of adjacent tiles.

Inputs

• absolute path of the XML descriptor (--projin)

Other inputs (optional)

• output XML descriptor filepath (--projout)
• reliability threshold (--threshold)

Output

• XML descriptor saved at --projout containing one displacement for each pair of adjacent tiles.

Demo (command line)

terastitcher --displthres --projin=C:/volume/xml_displproj.xml" --threshold=0.7

Demo (GUI)