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v1.2.0
Added
-
Add
-k/--max-hits Nto cap Bowtie2 alignments per read. -
Add
--all-hits(alias--full) to report all Bowtie2 alignments (-a), as earlier versions did. -
Keep the Bowtie2 alignments as BAM files in
<tmpdir>/bowtie/, written with--no-unal(unaligned reads are left out), so thresholds can be revisited without remapping. Use--no-bamto skip them.
Changed
- Add Read the Docs documentation (user guide, database, analysis, outputs, limitations, development) and shorten the README to point to it.
- Bowtie2 now reports at most 200 alignments per read by default (
-k 200). Benchmarks showed 0-1.1% of read assignments changing, unchanged detected taxa, and a much faster, lighter mapping step. Use--all-hitsfor the previous behaviour; results can differ slightly from 1.1.x otherwise. - The mapping step requests more memory (6x instead of 3x the input size) and time (3x) under
--all-hits, since uncapped runs ran out of memory or time at the capped request.