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Primer Scheme Correction #113

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moreiradaniel opened this issue May 13, 2021 · 1 comment
Closed

Primer Scheme Correction #113

moreiradaniel opened this issue May 13, 2021 · 1 comment

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@moreiradaniel
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Hi,

First of all, thanks for the effort to build and maintain the Signal workflow.

There is a small correction to be made (covid-19-signal/resources/primer_schemes/2kb_resende_v2/). I'm working with SARS-CoV-2 data from Resende's Lab and I noticed that the primer sequence (nCoV-2019_2_LEFT) described at https://doi.org/10.1101/2020.04.30.069039 aligns to reference (MN908947.3) from 1925 to 1947 (0-based), instead of 1916:1942.

Best,
Daniel

fmaguire added a commit that referenced this issue May 13, 2021
@fmaguire
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Thanks for looking so carefully at this!

The scheme in the repo is a modified version of the resende scheme by National Microbiology Lab to deal with a bit of dropout. I've just added in the original freede scheme from the paper with nCoV-2019_2_LEFT 1925:1947 so folks can find both.

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