Skip to content

v0.11.3

Latest
Latest

Choose a tag to compare

View all tags
@rafalkowalewski1 rafalkowalewski1 released this 01 Oct 10:41
v0.11.3
a1e639a
This commit was created on GitHub.com and signed with GitHub’s verified signature.
GPG key ID: B5690EEEBB952194
Verified
Learn about vigilant mode.

Picasso 0.11.3 is here

Changelog

  • Breaking change: dark times are now the number of frames without signal between binding events, i.e., one frame shorter than before (two binding events in consecutive frames have a dark time of 0). Together with the bright time, each now counts the frames spent in its state. This affects Render's pick info, qPAINT (picasso.postprocess.evaluate_picks, pick_kinetics and pick_properties) and picasso dark; influx rates calibrated with earlier versions should be recalibrated. See the documentation for the convention.

  • Localize: auto contrast button in the bottom right corner of the window; contrast dialog still exists for a numerical input.

  • Localize: new Identify on mode for multichannel and split-FOV data, Sum of unregistered channels. It adds the channels up (in photons) pixel for pixel, without any registration. See the documentation. The existing mode is renamed Sum of registered channels.

  • Localize: the channel sum has its own box size, min. net gradient and identification filters, the same whichever channel is displayed. Each channel keeps its own settings for identifying the channels separately (and for registering them for the sum).

  • Z binning for 3D calibration (Gaussian astigmatism and spline).

  • New picasso.localize.localize_frames: a GUI-free wrapper that runs the existing identification and fit on an in-memory frame stack (instead of a movie read from disk) and assigns absolute frame indices, so batched or live input concatenates into one growing localization table. Results are numerically identical to picasso.localize.localize on the same frames and parameters.

  • Fixed Localize not saying why the channel sum was not shown (channels not registered/aligned yet).

  • Render: NeNA is saved to metadata when calculated.

  • Fixed saving rotated localizations #708

  • Fixed .csv export in plot profile #709

  • Fixed Render's 3D slicer failing to open for some z ranges and for data thinner than one slice #710.

  • Fixed SPINNA single simulation without loading experimental data.

Notes

⚠️ Please see the readme.txt in the .zip files for important installation notes (camera config, Windows/macOS security warnings).

Assets 5
Loading