Alignment

Beam height above table surface: 160 mm

Set Up

1. Ensure that the alignment laser is level with the table by checking its height at two separate, far apart locations on the bench.
2. Ensure that the alignment laser is collimated. Below is a table of wavefront radii of curvature at different distances from the laser as measured with a Shack-Hartmann wavefront sensor.
3. Set the alignment laser beam height to ~160 mm above the table surface.
4. Ensure that the galvanometer mirror is at that correct height above the table by carefully putting the alignment laser next to it and visually verifying that it would intercept the entire cross section of the beam.

Distance, cm	RoC, m
50 cm	-30 +/- 1
75 cm	-35 +/- 2
100 cm	-40 +/- 3

Excitation Path

Phase 1

Relative Positioning of the Galvo/Scan Lens to Objective/Tube Lens Subsystems

1. Set the galvanometer voltage to 0V
2. Put an adjustable ND filter in front of the laser beam.
3. Place two irises on a row of bolt holes on the table. Align the laser to them.
4. Insert the galvanometer mirror and place it so that the beam is roughly in the center of the mirror.
5. Use C-clamps to move the two irises after the mirror on a column of bolt holes. Position the galvanometer so that the beam passes through the irises at these new positions.
6. Close down both irises. Insert a mirror after the second iris and align the back reflected beam back through both irises.
7. Insert a coverslip adter the ND filter to pick off the reflected beam.
8. Image the picked off beam on the Shack-Hartmann sensor. Mark its location. Be sure not to saturate the image.
9. Insert scan lens one (SL1) and place it such that the distance between the mirror and the lens housing is approximately 170 mm. Use the speckle size from scattering from a white piece of paper to verify that the mirror is near focus.
10. Adjust positioning of SL1 until the pick off beam is recentered on the camera and the forward the beam goes through both irises.