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BjornFJohansson edited this page Jun 3, 2024
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This wiki contains protocols and other information of interest to the mec group at CBMA and people working in the LGM lab.
You can use the search box at the right to search for page titles. Use this method to search inside the pages.
- Starting to work in the MEC group
- Maintaining lab notes
- How to mark your material
- Autoclaving
- Yeast and bacterial freezer stocks
- LGM
- Congresses
- Printable calendars
- Dept phone directory
- Antibiotics
- EGB24
- EGB2023 In vivo assembly in yeast results
- GBM23
- Yeast transformation GBM23
- GMB21
- In vivo assembly in yeast 2020
- LIB20
- Purifying and testing recombinant Taq DNA polymerase
- SimplyCloning
- How many times can I reuse electrophoresis buffer?
- TAE
- fatval
- Turner
- Bachman
- Midori Green DNA loading buffer (LBx2wMidori)
- LiAc Borate
- Boiling miniprep
- CAVAN low cost plasmid miniprep protocol without kit (super snik)
- Merlin
- Plasmid midiprep protocol
Chromosomal DNA / Total DNA / Genomic DNA / gDNA / plasmid DNA / pDNA preparation from S. cerevisiae
- Extraction of chromosomal DNA with PCA (phenol:chloroform:isoamylalcohol)
- Rapid isolation of yeast total DNA
- Yeast genomic DNA extraction with LiOAc SDS
- Yeast DNA isolation with Lyticase or Zymolyase
- Plasmid rescue to E. coli with plasmid miniprep kit
- bradford
- disintegration protocol
- pYPKa cloning protocol
- Transcription Unit cloning protocol
- Pathway assembly with the Yeast Pathway Kit
- sequencing
- Taq purification
- microwave yeast colony pcr
- Yeast cell fixation with formaldehyde
- LB
- LB-amp
- LB-kan
- LBpowder
- M9
- SOB (Super Optimal Broth)
- SOC (SOB with glucose)
- Super Broth
- Terrific Broth
- LB24
- 2xPCR mastermix
- Agar 40 g/L (2x)
- 40% w/v Glucose stock solution for media
- 40% w/v Sucrose stock solution for media
- 25% w/v NaCl stock solution for media
- 3.5M KCl stock solution.
- 2.5M MgCl2 stock solution.
- 2M MgSO4 stock solution.
- 0.5 M EDTA stock solution, pH 8
- 1.0 M TrisHCl, pH 8
- 1M IPTG stock solution
- 200 mg/mL amp icillin solution (x1000)
- 200 mg/mL Geneticin solution (x1000)
- 12.5 mg/mL Tetracycline solution (x1000)
- 10 mM dNTPs solution
- 5x Ligase buffer
- DNA DNA loading-buffer with FICOLL and two dyes.
- PBS