Hygromycin

Hygromycin B

Standard concentration for selection in S. cerevisiae is 300 µg/ml according to Goldstein 1999

Formedium sell this here.

Stock solution 300 mg/mL (x 1000)

• Weigh 3 g Hygromycin B powder in a Falcon 15 mL tube.
• Add water to the 10 mL mark and vortex. Add more water if necessary.
• Filter through 0.22 µM membrane with a large syringe.
• store at -20 in 1 ml aliquots.