E coli K12 MG1655 Resequencing

Instrument:  PacBio RS
Chemistry:  C2
Enzyme: C2

This Escherichia coli resequencing dataset showcases PacBio®'s extremely long-read, single molecule data with a sample of the E. coli K12 MG1655 strain.

Data consist of raw, unfiltered base files as well as data analysis with SMRT® Pipe, including filtered reads in FASTQ format, mapped reads in SAM/BAM, consensus calls and variants in VCF format.

Key metrics for sample data on v1.3.0 with C2 chemistry:

• 1 SMRT Cell, one 90 min. movie to maximize read length
• Consensus accuracy: 99.99% (Q40)
• Mean mapped read length: 3,549 bp
• 95th percentile mapped read length: 9,009 bp
• Coverage: 19.96x
• Mapped bases: 95.26 Mbp

Instrument Output (Primary Analysis) Data

• E. coli K12 MG1655 Raw Reads HDF 1.15 GB md5

Final (Secondary Analysis) Data

• E. coli K12 MG1655 Alignment FASTQ, SAM, VCF 767 MB md5