Microbial Multiplexing Data Set 48 plex: PacBio Sequel II System, Chemistry v2.0, SMRT Link v8.0 Analysis

SAMPLE

15 microbial isolates acquired from ATCC

• Bacillus cereus 971
• Bacillus subtilis W23
• Burkholderia cepacia
• Enterococcus faecalis OG1RF
• Escherichia coli K12
• Escherichia coli W
• Helicobacter pylori J99
• Klebsiella pneumoniae
• Listeria monocytogenes*
• Methanocorpusculum labreanum Z
• Neisseria meningitidis FAM18
• Rhodopseudomonas palustris
• Staphylococcus aureus*
• Staphylococcus aureus subsp. aureus
• Treponema denticola A

*Sequencing results differ significantly from published references of the named strains, indicating that these microbes are most likely mislabeled.

METHODS

• Microbial multiplexing protocol (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-Preparing-Multiplexed-Microbial-Libraries-Using-SMRTbell-Express-Template-Prep-Kit-2.0.pdf)
• Library prep: SMRTbell Express Template Prep Kit 2.0
• Sequencing: Sequel II System binding 2.0 kit (101-789-500) and Sequel II Sequencing 2.0 kit (101-820-200)
• Run time: 2 hour pre-extension; 15 hour movie
• Analysis Application: SMRT Link v8.0 Microbial Assembly

DOWNLOAD

Download Data: https://downloads.pacbcloud.com/public/dataset/MicrobialMultiplexing_48plex/