How to reduce the size of diamond.daa file

[vinoth-maker]

Hi! I started running the Diamond analysis after downloading the database and index file. I have a sample of paired-end reads with about 14 million reads, totaling 6 GB. After the Diamond analysis, I ended up with a 30 GB .daa file. Is this normal? How can I reduce the file size by adjusting parameters? I need to process multiple samples, and I'm concerned that the size of the file might be an issue when opening it in MEGAN. I used the following command for the analysis:
nohup diamond blastx
-d /home/diamond_db/nr
-q /home/anacmj_alunos/vino/test/trimming/E043_trimmed.fastq
-o /home/anacmj_alunos/vino/test/Diamond/E043_trimmed.fastq.daa
-f 100
--threads 20 &

Regards
Vino

[bbuchfink]

It is normal as the daa format is not very space efficient. You can try adjusting -k or use --top instead.