SplicingEngine

Pure Python alternative splicing analysis pipeline.

Features

• PSI (Percent Spliced In) calculation
• Differential splicing (combined t-test + Fisher's exact)
• RBP motif enrichment analysis
• 5 event types: SE, A5SS, A3SS, MXE, RI

Usage

pip install numpy scipy pandas matplotlib
python splicing_engine.py

Results (synthetic RNA-seq, 8 samples, 500 events)

• 60 differential splicing events (|dPSI|>0.1, FDR<0.05)
• Top event: ENASE0142 (dPSI=-0.39)
• Top RBP: SRSF1 motif GGAGG (enrichment=5.28, p<0.0001)