Region specific germline

[sikhlas]

As someone new to B cell immunoglobulin repertoire sequencing, my background is mainly in antibody production, I’m starting to draft a research proposal aiming to sequence B cells from the Saudi population to identify novel alleles and potentailly a region-specific germline dataset. However as a beginner, I’m unsure about how many human samples would be sufficient to provide robust information for constructing a population-representative germline database. Does anyone have recommendations or references regarding optimal sample sizes for this kind of immunogenetic study, especially when aiming to discover novel alleles or capture regional germline?
I am think of 75 samples of blood from healthy individuals from different sub-regions.