Release 0.9 #205

muffato · 2025-10-03T09:45:34Z

PR checklist

A quick patch to update busco 5.8 to busco 6

Update to wave containers

New new containers

Env update

Addition of resources for BLASTN_TAXON I have a clutch of assemblies which are all failing at this step due to memory. I've added a multiplier too so they don't end up in a constant cycle of life and death at the same mem requirement.

Damon should use his eyes more

@muffato

Update based on comments from @muffato

Dp24 taxon resources

Doc update

dp24-Busco-6-hotfix

Report BUSCO errors

Network resiliency

Comment out some lines which may be causing a channel race condition

…outside the closure

Blast busco

…nto add_tmp

Update to include tmpdir

sanger-tolsoft · 2025-10-03T09:46:07Z

Warning

Newer version of the nf-core template is available.

Your pipeline is using an old version of the nf-core template: 3.2.1.
Please update your pipeline to the latest version.

For more documentation on how to update your pipeline, please see the nf-core documentation and Synchronisation documentation.

github-actions · 2025-10-03T09:46:49Z

`nf-core pipelines lint` overall result: Passed ✅ ⚠️

Posted for pipeline commit c74dcea

+| ✅ 189 tests passed       |+
#| ❔  28 tests were ignored |#
!| ❗  19 tests had warnings |!

❗ Test warnings:

local_component_structure - create_bed.nf in modules/local should be moved to a TOOL/SUBTOOL/main.nf structure
local_component_structure - generate_config.nf in modules/local should be moved to a TOOL/SUBTOOL/main.nf structure
local_component_structure - nohit_list.nf in modules/local should be moved to a TOOL/SUBTOOL/main.nf structure
local_component_structure - windowstats_input.nf in modules/local should be moved to a TOOL/SUBTOOL/main.nf structure
local_component_structure - jsonify_taxdump.nf in modules/local should be moved to a TOOL/SUBTOOL/main.nf structure
local_component_structure - compressblobdir.nf in modules/local should be moved to a TOOL/SUBTOOL/main.nf structure
local_component_structure - restructurebuscodir.nf in modules/local should be moved to a TOOL/SUBTOOL/main.nf structure
local_component_structure - run_blastn.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
local_component_structure - minimap_alignment.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
local_component_structure - blobtools.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
local_component_structure - collate_stats.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
local_component_structure - view.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
local_component_structure - run_blastx.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
local_component_structure - prepare_genome.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
local_component_structure - coverage_stats.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
local_component_structure - finalise_blobdir.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
local_component_structure - busco_diamond_blastp.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
local_component_structure - input_check.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
included_configs - Pipeline config does not include custom configs. Please add the includeConfig line.

❔ Tests ignored:

files_exist - File is ignored: CODE_OF_CONDUCT.md
files_exist - File is ignored: assets/nf-core-blobtoolkit_logo_light.png
files_exist - File is ignored: docs/images/nf-core-blobtoolkit_logo_light.png
files_exist - File is ignored: docs/images/nf-core-blobtoolkit_logo_dark.png
files_exist - File is ignored: .github/ISSUE_TEMPLATE/config.yml
files_exist - File is ignored: .github/workflows/awstest.yml
files_exist - File is ignored: .github/workflows/awsfulltest.yml
files_exist - File is ignored: conf/igenomes.config
nextflow_config - Config variable ignored: manifest.name
nextflow_config - Config variable ignored: manifest.homePage
files_unchanged - File ignored due to lint config: CODE_OF_CONDUCT.md
files_unchanged - File ignored due to lint config: LICENSE or LICENSE.md or LICENCE or LICENCE.md
files_unchanged - File ignored due to lint config: .github/CONTRIBUTING.md
files_unchanged - File ignored due to lint config: .github/ISSUE_TEMPLATE/bug_report.yml
files_unchanged - File does not exist: .github/ISSUE_TEMPLATE/config.yml
files_unchanged - File ignored due to lint config: .github/PULL_REQUEST_TEMPLATE.md
files_unchanged - File ignored due to lint config: .github/workflows/branch.yml
files_unchanged - File ignored due to lint config: .github/workflows/linting_comment.yml
files_unchanged - File ignored due to lint config: .github/workflows/linting.yml
files_unchanged - File ignored due to lint config: assets/email_template.html
files_unchanged - File ignored due to lint config: assets/email_template.txt
files_unchanged - File ignored due to lint config: assets/sendmail_template.txt
files_unchanged - File ignored due to lint config: assets/nf-core-blobtoolkit_logo_light.png
files_unchanged - File ignored due to lint config: docs/images/nf-core-blobtoolkit_logo_light.png
files_unchanged - File ignored due to lint config: docs/images/nf-core-blobtoolkit_logo_dark.png
actions_awstest - 'awstest.yml' workflow not found: /home/runner/work/blobtoolkit/blobtoolkit/.github/workflows/awstest.yml
template_strings - template_strings
merge_markers - merge_markers

✅ Tests passed:

files_exist - File found: .gitattributes
files_exist - File found: .gitignore
files_exist - File found: .nf-core.yml
files_exist - File found: .editorconfig
files_exist - File found: .prettierignore
files_exist - File found: .prettierrc.yml
files_exist - File found: CHANGELOG.md
files_exist - File found: CITATIONS.md
files_exist - File found: LICENSE or LICENSE.md or LICENCE or LICENCE.md
files_exist - File found: nextflow_schema.json
files_exist - File found: nextflow.config
files_exist - File found: README.md
files_exist - File found: .github/.dockstore.yml
files_exist - File found: .github/CONTRIBUTING.md
files_exist - File found: .github/ISSUE_TEMPLATE/bug_report.yml
files_exist - File found: .github/ISSUE_TEMPLATE/feature_request.yml
files_exist - File found: .github/PULL_REQUEST_TEMPLATE.md
files_exist - File found: .github/workflows/branch.yml
files_exist - File found: .github/workflows/ci.yml
files_exist - File found: .github/workflows/linting_comment.yml
files_exist - File found: .github/workflows/linting.yml
files_exist - File found: assets/email_template.html
files_exist - File found: assets/email_template.txt
files_exist - File found: assets/sendmail_template.txt
files_exist - File found: conf/modules.config
files_exist - File found: conf/test.config
files_exist - File found: conf/test_full.config
files_exist - File found: docs/output.md
files_exist - File found: docs/README.md
files_exist - File found: docs/README.md
files_exist - File found: docs/usage.md
files_exist - File found: main.nf
files_exist - File found: assets/multiqc_config.yml
files_exist - File found: conf/base.config
files_exist - File found: conf/igenomes_ignored.config
files_exist - File found: modules.json
files_exist - File found: ro-crate-metadata.json
files_exist - File not found check: .github/ISSUE_TEMPLATE/bug_report.md
files_exist - File not found check: .github/ISSUE_TEMPLATE/feature_request.md
files_exist - File not found check: .github/workflows/push_dockerhub.yml
files_exist - File not found check: .markdownlint.yml
files_exist - File not found check: .nf-core.yaml
files_exist - File not found check: .yamllint.yml
files_exist - File not found check: bin/markdown_to_html.r
files_exist - File not found check: conf/aws.config
files_exist - File not found check: docs/images/nf-core-blobtoolkit_logo.png
files_exist - File not found check: lib/Checks.groovy
files_exist - File not found check: lib/Completion.groovy
files_exist - File not found check: lib/NfcoreTemplate.groovy
files_exist - File not found check: lib/Utils.groovy
files_exist - File not found check: lib/Workflow.groovy
files_exist - File not found check: lib/WorkflowMain.groovy
files_exist - File not found check: lib/WorkflowBlobtoolkit.groovy
files_exist - File not found check: parameters.settings.json
files_exist - File not found check: pipeline_template.yml
files_exist - File not found check: Singularity
files_exist - File not found check: lib/nfcore_external_java_deps.jar
files_exist - File not found check: .travis.yml
nextflow_config - Found nf-schema plugin
nextflow_config - Config variable found: manifest.nextflowVersion
nextflow_config - Config variable found: manifest.description
nextflow_config - Config variable found: manifest.version
nextflow_config - Config variable found: timeline.enabled
nextflow_config - Config variable found: trace.enabled
nextflow_config - Config variable found: report.enabled
nextflow_config - Config variable found: dag.enabled
nextflow_config - Config variable found: process.cpus
nextflow_config - Config variable found: process.memory
nextflow_config - Config variable found: process.time
nextflow_config - Config variable found: params.outdir
nextflow_config - Config variable found: params.input
nextflow_config - Config variable found: validation.help.enabled
nextflow_config - Config variable found: manifest.mainScript
nextflow_config - Config variable found: timeline.file
nextflow_config - Config variable found: trace.file
nextflow_config - Config variable found: report.file
nextflow_config - Config variable found: dag.file
nextflow_config - Config variable found: validation.help.beforeText
nextflow_config - Config variable found: validation.help.afterText
nextflow_config - Config variable found: validation.help.command
nextflow_config - Config variable found: validation.summary.beforeText
nextflow_config - Config variable found: validation.summary.afterText
nextflow_config - Config variable (correctly) not found: params.nf_required_version
nextflow_config - Config variable (correctly) not found: params.container
nextflow_config - Config variable (correctly) not found: params.singleEnd
nextflow_config - Config variable (correctly) not found: params.igenomesIgnore
nextflow_config - Config variable (correctly) not found: params.name
nextflow_config - Config variable (correctly) not found: params.enable_conda
nextflow_config - Config variable (correctly) not found: params.max_cpus
nextflow_config - Config variable (correctly) not found: params.max_memory
nextflow_config - Config variable (correctly) not found: params.max_time
nextflow_config - Config variable (correctly) not found: params.validationFailUnrecognisedParams
nextflow_config - Config variable (correctly) not found: params.validationLenientMode
nextflow_config - Config variable (correctly) not found: params.validationSchemaIgnoreParams
nextflow_config - Config variable (correctly) not found: params.validationShowHiddenParams
nextflow_config - Config timeline.enabled had correct value: true
nextflow_config - Config report.enabled had correct value: true
nextflow_config - Config trace.enabled had correct value: true
nextflow_config - Config dag.enabled had correct value: true
nextflow_config - Config dag.file ended with .html
nextflow_config - Config variable manifest.nextflowVersion started with >= or !>=
nextflow_config - Config manifest.version does not contain dev for release: 0.9.0
nextflow_config - Config params.custom_config_version is set to master
nextflow_config - Config params.custom_config_base is set to https://raw.githubusercontent.com/nf-core/configs/master
nextflow_config - Lines for loading custom profiles found
nextflow_config - nextflow.config contains configuration profile test
nextflow_config - Config default value correct: params.image_format= png
nextflow_config - Config default value correct: params.outdir= results
nextflow_config - Config default value correct: params.custom_config_version= master
nextflow_config - Config default value correct: params.custom_config_base= https://raw.githubusercontent.com/nf-core/configs/master
nextflow_config - Config default value correct: params.publish_dir_mode= copy
nextflow_config - Config default value correct: params.max_multiqc_email_size= 25.MB
nextflow_config - Config default value correct: params.validate_params= true
files_unchanged - .gitattributes matches the template
files_unchanged - .prettierrc.yml matches the template
files_unchanged - .github/.dockstore.yml matches the template
files_unchanged - .github/ISSUE_TEMPLATE/feature_request.yml matches the template
files_unchanged - docs/README.md matches the template
files_unchanged - .gitignore matches the template
files_unchanged - .prettierignore matches the template
actions_ci - '.github/workflows/ci.yml' is triggered on expected events
actions_ci - '.github/workflows/ci.yml' checks minimum NF version
readme - README Nextflow minimum version badge matched config. Badge: 24.04.2, Config: 24.04.2
readme - README Zenodo placeholder was replaced with DOI.
pipeline_todos - No TODO strings found
plugin_includes - No wrong validation plugin imports have been found
pipeline_name_conventions - Name adheres to nf-core convention
schema_lint - Schema lint passed
schema_lint - Schema title + description lint passed
schema_lint - Input mimetype lint passed: 'text/csv'
schema_params - Schema matched params returned from nextflow config
system_exit - No System.exit calls found
actions_schema_validation - Workflow validation passed: ci.yml
actions_schema_validation - Workflow validation passed: linting.yml
actions_schema_validation - Workflow validation passed: sanger_test_full.yml
actions_schema_validation - Workflow validation passed: download_pipeline.yml
actions_schema_validation - Workflow validation passed: linting_comment.yml
actions_schema_validation - Workflow validation passed: sanger_test.yml
actions_schema_validation - Workflow validation passed: template_version_comment.yml
actions_schema_validation - Workflow validation passed: branch.yml
actions_schema_validation - Workflow validation passed: fix-linting.yml
actions_schema_validation - Workflow validation passed: clean-up.yml
modules_json - Only installed modules found in modules.json
multiqc_config - assets/multiqc_config.yml found and not ignored.
multiqc_config - assets/multiqc_config.yml contains report_section_order
multiqc_config - assets/multiqc_config.yml contains export_plots
multiqc_config - assets/multiqc_config.yml contains report_comment
multiqc_config - assets/multiqc_config.yml follows the ordering scheme of the minimally required plugins.
multiqc_config - assets/multiqc_config.yml contains 'export_plots: true'.
modules_structure - modules directory structure is correct 'modules/nf-core/TOOL/SUBTOOL'
base_config - conf/base.config found and not ignored.
base_config - WINDOWSTATS_INPUT found in conf/base.config and Nextflow scripts.
base_config - BLOBTOOLKIT_WINDOWSTATS found in conf/base.config and Nextflow scripts.
base_config - FASTAWINDOWS found in conf/base.config and Nextflow scripts.
base_config - BUSCO_BUSCO found in conf/base.config and Nextflow scripts.
base_config - BLAST_BLASTN found in conf/base.config and Nextflow scripts.
base_config - NOHIT_LIST found in conf/base.config and Nextflow scripts.
modules_config - conf/modules.config found and not ignored.
modules_config - WINDOWMASKER_MKCOUNTS found in conf/modules.config and Nextflow scripts.
modules_config - WINDOWMASKER_USTAT found in conf/modules.config and Nextflow scripts.
modules_config - MINIMAP2_HIC found in conf/modules.config and Nextflow scripts.
modules_config - MINIMAP2_ILMN found in conf/modules.config and Nextflow scripts.
modules_config - MINIMAP2_CCS found in conf/modules.config and Nextflow scripts.
modules_config - MINIMAP2_CLR found in conf/modules.config and Nextflow scripts.
modules_config - MINIMAP2_ONT found in conf/modules.config and Nextflow scripts.
modules_config - MINIMAP2_ found in conf/modules.config and Nextflow scripts.
modules_config - SAMTOOLS_VIEW found in conf/modules.config and Nextflow scripts.
modules_config - SAMTOOLS_INDEX found in conf/modules.config and Nextflow scripts.
modules_config - PIGZ_COMPRESS found in conf/modules.config and Nextflow scripts.
modules_config - BLOBTK_DEPTH found in conf/modules.config and Nextflow scripts.
modules_config - BUSCO_BUSCO found in conf/modules.config and Nextflow scripts.
modules_config - RESTRUCTUREBUSCODIR found in conf/modules.config and Nextflow scripts.
modules_config - DIAMOND_BLASTP found in conf/modules.config and Nextflow scripts.
modules_config - DIAMOND_BLASTX found in conf/modules.config and Nextflow scripts.
modules_config - BLOBTK_DEPTH found in conf/modules.config and Nextflow scripts.
modules_config - BLOBTOOLKIT_WINDOWSTATS found in conf/modules.config and Nextflow scripts.
modules_config - BLOBTOOLKIT_CREATEBLOBDIR found in conf/modules.config and Nextflow scripts.
modules_config - BLOBTOOLKIT_UPDATEBLOBDIR found in conf/modules.config and Nextflow scripts.
modules_config - BLOBTOOLKIT_CHUNK found in conf/modules.config and Nextflow scripts.
modules_config - BLOBTOOLKIT_UNCHUNK found in conf/modules.config and Nextflow scripts.
modules_config - NOHIT_LIST found in conf/modules.config and Nextflow scripts.
modules_config - BLAST_BLASTN found in conf/modules.config and Nextflow scripts.
modules_config - COMPRESSBLOBDIR found in conf/modules.config and Nextflow scripts.
modules_config - BLOBTK_IMAGES found in conf/modules.config and Nextflow scripts.
modules_config - JSONIFY_TAXDUMP found in conf/modules.config and Nextflow scripts.
modules_config - MULTIQC found in conf/modules.config and Nextflow scripts.
nfcore_yml - Repository type in .nf-core.yml is valid: pipeline
nfcore_yml - nf-core version in .nf-core.yml is set to the latest version: 3.2.1
version_consistency - Version tags are numeric and consistent between container, release tag and config.

Run details

nf-core/tools version 3.2.1
Run at 2025-10-03 10:21:03

DLBPointon

Scyther LTGM!

DLBPointon and others added 30 commits July 15, 2025 16:37

dp24-Busco-6-hotfix

d149532

A quick patch to update busco 5.8 to busco 6

Update main.nf

e65ac06

Update to wave containers

Update main.nf

009de8e

New new containers

Update environment.yml

c2aaac3

Env update

Update base.config

c14c295

Addition of resources for BLASTN_TAXON I have a clutch of assemblies which are all failing at this step due to memory. I've added a multiplier too so they don't end up in a constant cycle of life and death at the same mem requirement.

Update base.config

54aa413

Damon should use his eyes more

Update base.config

118e8ca

Update based on comments from @muffato

Merge pull request #191 from sanger-tol/dp24-TAXON-resources

da0ed54

Dp24 taxon resources

Test data have moved off /lustre/scratch123

90e88a5

Updates post nf-core merge

b877814

Ignore environment.yml from prettier linting

4b8034b

Update busco, thanks to muffato's prettier update to busco in nf-core

06ec96c

URLs should be protected with <>

a2d9443

Added missing citations

4361fa3

Docs update

75b125d

Added Damon to the list of contributors

84c9dfd

Corrected the initial version of Busco

eb24495

Merge pull request #193 from sanger-tol/docs

f931a14

Doc update

Merge pull request #190 from sanger-tol/dp24-busco-6-patch

385c1f1

dp24-Busco-6-hotfix

Busco module update (stop ignoring errors)

1bba35c

Merge pull request #194 from sanger-tol/busco_errors

936cfbc

Report BUSCO errors

Edited for consistency

238d198

More time to allow for larger genomes

e805a38

Version bump

ecad033

Rolled out the session-retry mechanism to all API calls

b001c94

Changelog update

10840b1

Release name !

dce9497

Merge pull request #197 from sanger-tol/network_resiliency

97931d6

Network resiliency

Update to include tmpdir

49fe8a1

Update changelog

29d3bc7

DLBPointon and others added 16 commits September 19, 2025 13:57

Comment out some lines which may be causing a channel race condition

7e9ca92

Merge pull request #201 from sanger-tol/fix_channel_race

1a5251b

Comment out some lines which may be causing a channel race condition

Correction based on comments from @muffato

cba18cc

linter!

c2dd92e

Changelog

427a6bd

In this pipeline we are happy with Prodigal failing

4bd86b8

Increase quadratically to waste less time on retries

ab502c7

Alternative implementation that doesn't rely on accessing a variable …

6a31a21

…outside the closure

Merge pull request #202 from sanger-tol/blast_busco

99d807d

Blast busco

Updates based on comments

82b9890

Updates based on comments

f134a96

Merge branch 'dev' into add_tmp

d7d45bd

Darn spelling mistakes!

cc150bd

Merge branch 'add_tmp' of https://github.com/sanger-tol/blobtoolkit i…

a40c7fd

…nto add_tmp

Darn spelling mistakes!

a468982

Merge pull request #200 from sanger-tol/add_tmp

54a4b37

Update to include tmpdir

New release date

c74dcea

muffato requested a review from DLBPointon October 3, 2025 10:20

muffato self-assigned this Oct 3, 2025

This was linked to issues Oct 3, 2025

Problems running v0.8.0 using nextflow v0.25 (in nf-core-3.2.1-0) #185

Closed

test is not working as expected. Fails on generate config #196

Closed

DLBPointon approved these changes Oct 3, 2025

View reviewed changes

muffato merged commit 3c9edea into main Oct 3, 2025
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Release 0.9 #205

Release 0.9 #205

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muffato commented Oct 3, 2025

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sanger-tolsoft commented Oct 3, 2025

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edited

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✅ Tests passed:

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muffato commented Oct 3, 2025

PR checklist

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sanger-tolsoft commented Oct 3, 2025

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nf-core pipelines lint overall result: Passed ✅ ⚠️

❗ Test warnings:

❔ Tests ignored:

✅ Tests passed:

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github-actions bot commented Oct 3, 2025 •

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`nf-core pipelines lint` overall result: Passed ✅ ⚠️