Make 2p raw data access notebook

[rcpeene]

I should make a notebook that describes how to use ZSTD compression, mentioned here

[jeromelecoq]

The goal of this notebook would be to:

• Show how to access raw data
• How to stream from Dandi the raw data. Handle decompression in the background if needed.
• How to visualize raw data frames
• Test NWB Widget with Raw data.
• How to overlay segmented cells on top of it.
• How to visualize associated calcium traces.

[rcpeene]

The best way to visualize the 3d raw 2p data will probably require mpl_interactions library. Issue #60 will include this.

[rcpeene]

It seems as though users won't need to handle decompression, and neither do I! As far as I can tell, decompression is handled by PyNWB automatically when the file is read.

[jeromelecoq]

Example view in this paper : https://www.nature.com/articles/nn.3867

[jeromelecoq]

@jeromelecoq and @rcpeene will review NWB regions data together

[jeromelecoq]

We discussed. @rcpeene was starting to put together a comprehensive real time view of the movies along with traces. This might be a separate effort. We will focus on static representations for now for this notebook.

[jeromelecoq]

We need to make sure the notebook showcase the time of each frame in the raw data. @Ahad-Allen will provide how to find this info.

[Ahad-Allen]

I believe this information is stored here in the NWB:

You should be able to map the frametimes from the raw to these to get the exact timings.

[rcpeene]

A basic version of this notebook has been pushed to its own branch visualize_2p_raw. Will issue a PR into dev when the current PR from dev to main is closed.

[jeromelecoq]

@rcpeene to link PR here

[jeromelecoq]

Ok, first the branch run on Binder. This is great!