This repository includes the Python scripts of the NovoBoard evaluation framework for de novo peptide sequencing. The scripts include two main parts:

• calculation of the fragment ion, amino acid, and peptide accuracies;
• generating decoy spectra, FDR estimation, and FDR validation.

Example ABRF dataset can be downloaded via the following link: https://drive.google.com/drive/folders/1_6azR4-YjTUfRYdsXbFhZL9lFvjdrIDh?usp=sharing

Paper: "NovoBoard: a comprehensive framework for evaluating the false discovery rate and accuracy of de novo peptide sequencing" (https://doi.org/10.1101/2024.04.16.589668)