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Wire M-P coculture GEM paper into Methylomicrobium-Chlorella community#63

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Wire M-P coculture GEM paper into Methylomicrobium-Chlorella community#63
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Summary

`doi:10.20517/mrr.2023.70` (Badr/He/Wang 2023, Microbiome Research Reports, "Probing interspecies metabolic interactions within a synthetic binary microbiome using genome-scale modeling") is an orphan cache that develops three GEM approaches — SteadyCom, dynamic FBA Lab, and the authors' new `DynamiCom` — on a methanotroph-photoautotroph (M-P) coculture model system.

Wire as PARTIAL evidence on the Methane and Carbon Dioxide Mitigation cross-feeding interaction in the existing `Methylomicrobium-Chlorella Methane Sequestration Coculture` community:

  • Same kind of methanotroph-microalga consortium as the curated record.
  • The abstract directly speaks to "the metabolic interactions within the coculture, how they contribute to the enhanced growth observed in the coculture" — central claim of that interaction.

PARTIAL because the modeled M-P system in the paper is generic, not the specific M. alcaliphilum 20Z + Chlorella HS2 pairing curated here.

Test plan

  • `just validate kb/communities/Methylomicrobium_Chlorella_Methane_Sequestration_Coculture.yaml` — no schema issues
  • `just validate-references kb/communities/Methylomicrobium_Chlorella_Methane_Sequestration_Coculture.yaml` — passes (snippet uses `..` splitter on two verbatim substrings of the abstract)

🤖 Generated with Claude Code

…ella

doi:10.20517/mrr.2023.70 (Badr/He/Wang 2023, "Probing interspecies
metabolic interactions within a synthetic binary microbiome using
genome-scale modeling") is an orphan cache that develops three
genome-scale modeling approaches - SteadyCom, dynamic FBA Lab, and
their new DynamiCom - on a methanotroph-photoautotroph (M-P)
coculture model system. Wire it as PARTIAL evidence on the
Methane-and-Carbon-Dioxide-Mitigation cross-feeding interaction in
the existing Methylomicrobium-Chlorella community: same kind of
methanotroph-microalga consortium as the curated record, and the
abstract directly speaks to "the metabolic interactions within the
coculture, how they contribute to the enhanced growth observed in
the coculture" - which is the central claim of that interaction.

PARTIAL because the modeled M-P system in the paper is generic
rather than the specific M. alcaliphilum 20Z + Chlorella HS2
pairing curated here.

Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com>
Copilot AI review requested due to automatic review settings May 17, 2026 07:47
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Pull request overview

This PR adds a new publication (doi:10.20517/mrr.2023.70) as PARTIAL, COMPUTATIONAL evidence to the existing Methylomicrobium–Chlorella Methane Sequestration Coculture community, aiming to support the “Methane and Carbon Dioxide Mitigation” cross-feeding interaction using genome-scale modeling results from a related (generic) methanotroph–photoautotroph coculture system.

Changes:

  • Adds a new evidence item referencing doi:10.20517/mrr.2023.70 under the “Methane and Carbon Dioxide Mitigation” interaction.
  • Introduces a multi-fragment abstract snippet (using ..) and an explanation describing the paper’s modeling-based support as partial.

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Comment thread kb/communities/Methylomicrobium_Chlorella_Methane_Sequestration_Coculture.yaml Outdated
Comment thread kb/communities/Methylomicrobium_Chlorella_Methane_Sequestration_Coculture.yaml Outdated
- Move the GEM-modeling evidence off the Methane-and-Carbon-Dioxide-
  Mitigation interaction and onto Saline-Metabolic-Coupling-and-pH-
  Recovery. The paper's central claims are about metabolic coupling
  driving enhanced coculture growth, not CH4/CO2 mitigation or
  biomass carbon sequestration; the new placement aligns the
  evidence with what the snippet actually supports ("robust
  metabolic coupling between the two microbes" is the lead evidence
  on this interaction).
- Author/year correction: "Badr/He/Wang 2023" -> "Badr/He/Wang 2024"
  to match the journal's 2024-05-27 publication date in Crossref
  (the "2023" in the DOI path 10.20517/mrr.2023.70 is the
  volume/issue stamp, not the publication year).

Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com>
@realmarcin realmarcin merged commit 4e80d0f into main May 17, 2026
@realmarcin realmarcin deleted the claude/orphan-curation-batch5 branch May 17, 2026 07:58
realmarcin added a commit that referenced this pull request May 18, 2026
* Regenerate community HTML and UMAP

Refresh docs/ to reflect community additions and evidence updates
since the last regen (PR #54). UMAP grows from 252 -> 261 embedded
communities; 7 existing community pages are refreshed with the
evidence additions from PRs #55, #58, #60, #61, #62, #63, #66, #67;
9 new community pages are added (2 from this session: Alaska Tundra
Permafrost Iron-Redox and Saccharomyces-Acinetobacter Lignocellulose
Detox; 7 from other curation work merged in between).

- gen-html: 264 community pages rendered without error
- gen-umap: 261 communities embedded (3 host-only skipped because
  none of their taxa have NCBITaxon embeddings)

Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com>

* Address Copilot review on PR #71: render evidence everywhere it lives

Three template/data fixes triggered by the Copilot review on the
HTML/UMAP regen:

- src/communitymech/templates/community.html:
  - Extend the interaction-network color map and legend with
    PREDATION, NICHE_PARTITIONING, STRAIN_COMPETITION, and
    COLONIZATION_FACILITATION. Previously these enum values fell
    back to generic gray on the force-directed graph and were
    omitted from the legend, making 5+ community pages
    (Cellulomonas-Rhodobacter, Sphingobium-Rhodococcus,
    Clostridium-Carboxidivorans-Kluyveri, Rhodopseudomonas-
    Geobacter-Magnetite, plus any others) hard to read.
  - Render evidence on taxonomy entries via an "evidence-row"
    span. The previous template only rendered evidence inside
    ecological_interactions and growth_media, so evidence wired
    onto taxon blocks in PRs like #61 and #62 was on-disk in the
    YAML but invisible in the rendered HTML.
  - Render evidence on environmental_factors with the same
    expanding-row pattern. This is where PR #62 wired the
    Ter Horst 2021 peat-virome evidence on the SPRUCE community;
    it now appears in the HTML.
  - Add an "External Resources" section (analogous to Associated
    Datasets) that renders external_resources entries including
    their per-resource evidence. This is where PR #66 wired the
    Smith 2025 BioModels reproducibility paper on the Kefir
    BioModels community; it now appears in the HTML.

- kb/communities/Alaska_Tundra_Permafrost_Iron_Redox_Community.yaml:
  add `metals_present: [IRON]`, `metal_relevance: SIGNIFICANT`,
  and metal_notes describing the Fe(III) reduction / Fe(II)
  oxidation cycle this community is built around. The community
  was previously absent from the iron/metal index filter despite
  being entirely iron-centric.

- docs/: regenerate all 264 community pages with the new template
  + the Alaska_Tundra metals metadata. Many pages pick up the new
  evidence-row block; the four pages with NICHE_PARTITIONING
  interactions now render those nodes with the dedicated color
  and include the new legend entries.

Verified after regen:
- SPRUCE community page now shows PMID:38515239 and PMID:34836550
  (zero matches before, two each after).
- Kefir BioModels community page now shows PMID:41343683 (zero
  matches before, two after).

Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com>

---------

Co-authored-by: Claude Opus 4.7 (1M context) <noreply@anthropic.com>
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2 participants