Multi exponential fit of D0 sample

Starting

Start ChiSurf by double-clicking on the icon. This might take a while.

File parameters

In the Start-Window click in the box next to “File parameters”. Change the default values: dt = 0.0563, rep. rate = 1 MHz.

Other options:

• Rebin: for high-resolution data, the histogram data can be rebinned, e.g. data collected at a dt of 4 ps can be changed to 16 ps binning by a 4fold rebinning.
• VV/VH-Jordi: mainly for single-molecule data, where one detector is placed parallel and the other is placed vertically to exciting laser beam of the microscope. Here, the data file contains first the decay in VV direction, followed by a blank line, and subsequently the VH data. The VM decay used for fitting is reconstructed in ChiSurf based on (I) the experimentally determined g-factor and (II) Japanese (lens) correction factor correcting for the un-ideality of the lenses/optical pieces in the beam path.
• Csv/ fixed width: In this section you can define your data format, if it is different than the IBH or PQ format

Concerning dt:

• Check your data files, what is “x”, the channel number (standard IBH) or the time (PQ format). If x = time, leave dt unclicked!!! The software then knows, that the first column in your data is the time. If you click on dt and specify a value, it assumes that x = channel number and multiplies your x-values with the specified dt.

Loading data

Load your data either by (I) Drag-and-drop or by clicking on “add data”. Generally, I prefer to load the IRF first, then D0, then DA. If your data path is quite long, you can adjust the width of the data/analysis pane, by dragging to the right.

In the next steps, you will need three data files from data set 1: “IRF_D”, “D0”, and “DA”.