Structural zeros and multi group comparisons #303
Unanswered
MarieBraadLund
asked this question in
Q&A
Replies: 0 comments
Sign up for free
to join this conversation on GitHub.
Already have an account?
Sign in to comment
Uh oh!
There was an error while loading. Please reload this page.
-
I have been running ancombc2 where I compare three groups A, B, and C. I wish to make all three comparisons (A-B, A-C, and B-C) and have run the code with and without the 'pairwise' option to obtain this. Regardless if I run it with or without the 'pairwise' option I run into the same problem with structural zeros:
If taxon_1 has structural zeros in group A, but is present in group B and C, then taxon_1 is not included in the differential abundance analysis. This makes sense when I compare group A and B or group A and C, but I don't think it makes sense when I compare group B and C where taxon_1 is present in both groups. However, this is what happens: taxon_1 is not present in the res output if it has structural zeros in just one of the three groups.
Is there an option to solve this issue? or do I have to run ancombc2 on two groups at a time to get the full analysis done?
Beta Was this translation helpful? Give feedback.
All reactions