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Sobel Filter + Markers + Watershed Transform (Seems like the best option so far -- without empirical validation)
Histogram-Based segmentation -- what amounts to "Bump hunting" and cutting on modes
There are many choices of filters that will pick out edges. Both Sobel and Canny use a convolution kernel which essentially calculates derivatives of gray scale values in the 8 cardinal directions.
We might want to think about:
Preprocessing
Denoising
Smoothing with a Gaussian or "Disk Median"
Morphological Opening and Closing to get rid of scattered members in contiguous regions