Angular_discrete_assign_v3
Make a projection assignment using wavelets on a discrete library of projections This program assigns Euler angles to experimental projections by matching with ideal projections. This matching is done via a DWT correlation. For every experimental projection, different in-plane rotations and shifts are tried (by exhaustive search). For each possible combination of these two variables, the best correlating ideal projection is sought using a fast multirresolution algorithm. The method is fully described at http://www.ncbi.nlm.nih.gov/pubmed/15099579
Parameters
--mode <modeoverwrite> where <mode> can be:
-
- $
-o, --output <output_file> $``: Metadata with the reference images and their angles Must be created withAngular_project_library_v3 $--sym <symmetry_file> =: Symmetry file if any The definition of the symmetry is described atTransform_symmetrize_v3$`--max_shift_change <r`0> $ `--psi_step <ang`5> $`--shift_step <r`1>
Extra parameters $: Perform a 5D search instead of 3D+2D $`--max_proj_change <ang`-1> $`--max_psi_change <ang`-1> $`--keep <th`50> $`--smin <s`1> $`--smax <s`-1> $`--pick <mth`1> $: Show the rot-tilt process $: Show the psi-shift process $: Show final options among which the angles are selected
Typical use:
xmipp_angular_project_library -i referenceVolume.vol -o reference.stk --sampling_rate 5 xmipp_angular_discrete_assign -i projections.sel -o discrete_assignment.xmd --ref reference.doc