This repository contains code and documentation for the analysis of gut microbiota composition in patients with Restless Legs Syndrome (RLS), insomnia (INS), and healthy controls (CTRL). The project is part of a research study exploring microbiota alterations across different RLS phenotypes using 16S rRNA sequencing and downstream statistical tools.
To analyze the fecal microbiota composition in patients with idiopathic Restless Legs Syndrome (RLS) and investigate its relationship with different RLS clinical features.
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Participants:
- 37 RLS patients (28 females, mean age: 64.78 years)
- 31 INS patients (22 females, mean age: 60.64 years)
- 33 CTRL individuals (24 females, mean age: 62.54 years)
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Microbiota Analysis:
- Sample collection: Stool samples collected and processed.
- Sequencing: 16S rRNA gene sequencing using Illumina MiSeq platform.
- Pipeline:
- DADA2 for sequence preprocessing and ASV table generation
- DESeq2 and ALDEx2 for differential abundance analysis
- Correction for age, sex, BMI, sequencing run, and presence of mood disorders
├── 0_Preprocessing_iRLS2_Metagenomics_OK.R
├── 1_iRLS2_Phyloseq_PhylogeneticTree_OK.R
├── 2_iRLS2_Metagenomics_Shrink_BigDataAnalysis_OK
│ ├── 3_iRLS_Metagenomica_TreatmentComparison_OK.R
│ ├── 4_20240724_iRLS_BDI_Analysis_OK.R
│ ├── 5_iRLS2_INSB_Metagenomics_Analysis_Family_OK.R
│ ├── 6_iRLS_Metagenomica_PHYLUM_OK.R
│ ├── 7_iRLS_Metagenomics_ALDEx2.R
│ ├── 8_iRLS2_picrust2_OK.R
├── 9_iRLS2_A_Diversity_OK.R
└── 10_iRLS2_B_Diversity_OK.R